Sequences from confirmation amplicon


Confirmation sequences for line 092C06 and hit At5g11750 are shown.
Line ID 092C06
Locus At5g11750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3787228
Primers used for PCR 03sw - 8474, show primer details
   
Sequence Name 25-K116770-0022-092-C06C-03sw
GenBank Accession LN497621 [GenBank]
Sequencing primer gene specific primer, 03sw: 5'-TGTCAGCGAGTTTCTCAGAAGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K116770-0022-092-C06C-03sw
ACTCTTGGGTCTTGATTGTTTAATCAAAAGAGAAAGGGTATGTTTTGATATAACCATAAA
AAGGAAGGGTATGTATGGAATTATGAAAGAAGAAAAAAGAGGATGTATTTGATGTAAGCA
TAAAACATCAGGGGTTTATATGCAATTACCAGGAAGAAAAGAAAAAAAGAATCCTTCAAC
GAGGGTTTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCACGATTATTATAAT
   
Sequence Name 01-K116769-0022-092-C06C-8409
GenBank Accession LN486741 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116769-0022-092-C06C-8409
ATTTACATTGATATATCCTGCAAACCCTCGTTGAAGGATTCTTTTTTTCTTTTCTTCCTG
GTAATTGCATATAAACCCCTGATGTTTTATGCTTACATCAAATACATCCATCTTTTTTCT
TCTTTCATAATTCCATACATACCCTTCCTTTTTATGGTTATATCAAAACATACCCTTTCT
CTTTTGATTAAACAATCAAGACCCAAGAGTCAGGAGTAATGAAATAGATTCTAGTATTTG
TCATATCTTCTGAGAACTTCGCTGACA

Last Updated on 06.12.2018 16:11