Genotyping and primer details


Genotyping and primer details for 092C06 - At5g11750
Line ID 092C06
Locus At5g11750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3787228 of line 092C06 Chr5:3787228
   
Insertion allele amplicon 8474 - 03sw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03sw: 5'-TGTCAGCGAGTTTCTCAGAAGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3786984
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11