Sequences from confirmation amplicon


Confirmation sequences for line 092G09 and hit At5g06860 are shown.
Line ID 092G09
Locus At5g06860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2133232
Primers used for PCR 03xb - 3144, show primer details
   
Sequence Name 53-K119006-0022-092-G09C-03xb
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 03xb: 5'-CGTTACCTAAAATCTTGGCTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K119006-0022-092-G09C-03xb
ATCTTTTCCTTTATTACATCATTCAGTGTCTTGTCTAAACAACGACTTTTTATGTTGTAG
GTTCCATACCAGAGTCATTTGGGTCGTTTCCAGGAACAGTCCCTGACCTTCGCCTATCAC
ATAACCAGCTCTCCGGTCCAATACCCAAATCATTAGGCAACATCGACTTTAACCGGATCG
ATTTATCCCGCAACAAGCTCCAAGGTGATGCTTCGATGTTGTTTGGTTCCAACAAAACAA
CCTGGTCTATTGACTTATCAAGAAACATGTTCCAGTTCGATATCTCAAAGGTTGATATCC
CTAAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCT
GTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGG
GATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCAC
   
Sequence Name 23-K119005-0022-092-G09C-35St
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K119005-0022-092-G09C-35St
AGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGACCTTTAGGGTACCCGGGGATC
AGATTGTCGTTTCCCGCCTTCTGTTTAAACTATCAGTGTTTTAGGGATATCAACCTTTGA
GATATCGAACTGGAACATGTTTCTTGATAAGTCAATAGACCAGGTTGTTTTGTTGGAACC
AAACAACATCGAAGCATCACCTTGGAGCTTGTTGCGGGATAAATCGATCCGGTTAAAGTC
GATGTTGCCTAATGATTTGGGTATTGGACCGGAGAGCTGGTTATGTGATAGGCGAAGGTC
AGGGACTGTTCCTGGAAACGACCCAAATGACTCTGGTATGGAACCTACAACATAAAGATC
GTTGTTTAGACAAGACACTGAATGATGTAATAAAGGAAAAGATCGTTCTTTACCTGTAAG
TTTGTTCCTGCTAAGTTCAAGAGCCAAGATTTTAGGTAACG

Last Updated on 06.12.2018 16:11