Sequences from confirmation amplicon


Confirmation sequences for line 094C05 and hit At3g61390 are shown.
Line ID 094C05
Locus At3g61390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22717587
Primers used for PCR 40fr - 8474, show primer details
   
Sequence Name 18-K111563-0022-094-C05C-40fr
GenBank Accession LN494363 [GenBank]
Sequencing primer gene specific primer, 40fr: 5'-GACAAATAAAGTATTGTGGCGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K111563-0022-094-C05C-40fr
CTCTTCTCGCGCTACCCTTCAGCTTTGATACTTCTCTAAGCTTTATCTTCAACTCTTCTT
GTTCTTCTCTGATTCCTCTAAACGTTTCCATTGATAAGTTGTTCTTTTCTTGAAGCTTTC
TAATCACAGTATAAGATTCGGTTATTTGGGTCTCGGATTCTGATCTCATCTTTTCAAGTT
CGTCATTTCTTTTCCTCAGTGCCTTCTCTGTTTCTTTCCTCTGCTTCAACTCCTCAAAAT
ACACATTTTCCCATTGTTTAGCCTACAATTGTTTTTGTTATAGATAAGAGTTAGAAAAGT
TTGATACTTCTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGC
   
Sequence Name 02-K111556w-0022-094-C05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K111556w-0022-094-C05C-8409
AGTATCAACTTTTCTAACTCTTATCTATACAAAAACAATTGTAGGCTAAACAATGGGAAA
ATGTGTATTTTGAGGAGTTGAAGCAGAGGAAAGAAACAGAGAAGGCACTGAGGAAAAGAA
ATGACGAACTTGAAAAGATGAGATCAGAATCCGAGACCCAAATAACCGAATCTTATACTG
TGATTAGAAAGCTTCAAGAAAAGAACAACTTATCAATGGAAACGTTTAGAGGAATCAGAG
AAGAACAAGAAGAGTTGAAGATAAAGCTTAGAGAAGTATCAAAGCTGAAGGGTAAGCGCG
AGGAAGAGGAAGCTTCTACCTCTAACCACCGTGAACCGCCACAATACTTTATTTGT

Last Updated on 06.12.2018 16:11