Sequences from confirmation amplicon


Confirmation sequences for line 094D07 and hit At5g51820 are shown.
Line ID 094D07
Locus At5g51820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21066378
Primers used for PCR KM79 - 8474, show primer details
   
Sequence Name 68-K038031-022-094-D07C-KM79
GenBank Accession LN511955 [GenBank]
Sequencing primer gene specific primer, KM79: 5'-AAATAATAGGACCATCAAAACCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K038031-022-094-D07C-KM79
CTATTTGGCTCTAGATGTAAGAACATACCCAAAATCTGATCTTGAAAGAAGACCTCTGAT
GAGATCAAAGTCGAAAACATCCTGCATAGAATACCATGAACATATCTAATGTCTAACATA
CCCAATATCATAAGACAAAAACAGAAGAATCATGTGAGAAAATTCTTACCTCCATGAGCT
CCAAATAGTCGGAAACAGGATCTATAACTTCTACACTGAAATTCCCATATTTGGTCACTC
CAACTTGAGAAAGATCAATATCAGGAATCTCTGCCACCTTAATCTCAGAGATCTGAAAAT
GTGTACCAGAGACGAGACCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 12-K038031-022-094-D07C-8409
GenBank Accession LN491489 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K038031-022-094-D07C-8409
CCGGACTGAGCCATTTCAATTGAATATGGTCTCGTCTCTGGTACACATTTTCAGATCTCT
GAGATTAAGGTGGCAGAGATTCCTGATATTGATCTTTCTCAAGTTGGAGTGACCAAATAT
GGGAATTTCAGTGTAGAAGTTATAGATCCTGTTTCCGACTATTTGGAGCTCATGGAGGTA
AGAATTTTCTCACATGATTCTTCTGTTTTTGTCTTATGATATTGGGTATGTTAGACATTA
GATATGTTCATGGTATTCTATGCAGGATGTTTTCGACTTTGATCTCATCAGAGGTCTTCT
TTCAAGATCAGATTTTGGGTATGTTCTTACATCTAGAGCCAAATAGGCTAGAAGCTTTTG
TCTTTTTTGGGGTTTTGATGGCCTTATTAT

Last Updated on 06.12.2018 16:11