Sequences from confirmation amplicon


Confirmation sequences for line 095A11 and hit At3g27020 are shown.
Line ID 095A11
Locus At3g27020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9962771
Primers used for PCR 11pi - 8474, show primer details
   
Sequence Name 35-K115005-0022-095-A11C-11pi
GenBank Accession LN501941 [GenBank]
Sequencing primer gene specific primer, 11pi: 5'-GTGGAAATATTATTGGGATTGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K115005-0022-095-A11C-11pi
GCATCGCAATTCAGGGGTATACGGTCCTTCAGAACACTTCATCTCTTTTCTTCTTCACCA
GCAGTATCTCAGAAGCTTCACTGTCATCTGAATTACGGCATTGGAAAACATACAGTTAAC
AGTCTGTGGAAAGTGCCCATGAGAGGATCCAAGTTATTGGTTAATACAAAAGCATGTAAG
AGATGAGTCAGGATCTAATATTCTCTCACCTACACCGTCGGTAACAATGGGTAGATTGAG
TCGTCTAGACCTGCTGCTGCATAATTCCTTCACAGTGACAGCGATGATCTTGACAAGATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
GATTATA
   
Sequence Name 03-K115004-0022-095-A11C-8409
GenBank Accession LN487604 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115004-0022-095-A11C-8409
TCTTGTCAGATCATCGCTGTCACTGTGAAGGAATTATGCAGCAGCAGGTCTAGACGACTC
AATCTACCCATTGTTACCGACGGTGTAGGTGAGAGAATATTAGATCCTGACTCATCTCTT
ACATGCTTTTGTATTAACCAATAACTTGGATCCTCTCATGGGCACTTTCCACAGACTGTT
AACTGTATGTTTTCCAATGCCGTAATTCAGATGACAGTGAAGCTTCTGAGATACTGCTGG
TGAAGAAGAAAAGAGATGAAGTGTTTCTGAAGGACCGTATACCCCTTGAATTTGCGATTG
CTGGTTATGTGGGTCTTGCAGCTATCTCAACTGCCACAATCCCAATAATATTTCCA

Last Updated on 06.12.2018 16:11