Sequences from confirmation amplicon


Confirmation sequences for line 095G03 and hit At5g33370 are shown.
Line ID 095G03
Locus At5g33370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:12604507
Primers used for PCR BD35 - 8474, show primer details
   
Sequence Name 05-K028144-022-095-G03C-8409
GenBank Accession LN488215 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K028144-022-095-G03C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATTACCCTTATGGTATCGATTTC
CCAACTCATCGTCCTACGGGCCGTTTCTCCAATGGTCTAAACATTCCAGATCTCATCAGT
AAGCTTTTAATTTCTTGTTCCCCAAGAAAATGAACACTCAACGTTACCAAAATTACATTA
ATAATAACAATGATCTTTTATAGGTGAACATTTGGGTCAAGAGTCTCCAATGCCGTACCT
TAGTCCAATGCTGAAGAAGGACAAACTCTTAAGGGGAGCTAACTTCGCCTCCGCGGGTAT
TGGAATCCTTAACGACACCGGAATCCAGTTTGTAAGTTCAACCGGAATAAATCTTACCAT
CGAGTTAACGGCATTCTTAAAATCTTGATGCTAAGAGGTGTTTTGACAATTTGAATGCAG
CTGAATATTATTAGGATCACAAAGCAGCTAGAGTACTTCGAGCAGTACAAGGTTCGAGTA
AGCGGGTTGGTCGGAGAAGAAGAGATGAATCGGCTCGTGAACGGAGCTCTT
   
Sequence Name 06-K028144-022-095-G03C-BD35
GenBank Accession LN488667 [GenBank]
Sequencing primer gene specific primer, BD35: 5'-TGTTATTAGGACAAGAGCTCCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K028144-022-095-G03C-BD35
GCCGATTCATCTCTTCTTCTCCGACCAACCCGCTTACTCGAACCTTGTACTGCTCGAAGT
ACTCTAGCTGCTTTGTGATCCTAATAATATTCAGCTGCATTCAAATTGTCAAAACACCTC
TTAGCATCAAGATTTTAAGAATGCCGTTAACTCGATGGTAAGATTTATTCCGGTTGAACT
TACAAACTGGATTCCGGTGTCGTTAAGGATTCCAATACCCGCGGAGGCGAAGTTAGCTCC
CCTTAAGAGTTTGTCCTTCTTCAGCATTGGACTAAGGTACGGCATTGGAGACTCTTGACC
CAAATGTTCACCTATAAAAGATCATTGTTATTATTAATGTAATTTTGGTAACGTTGAGTG
TTCATTTTCTTGGGGAACAAGAAATTAAAAGCTTACTGATGAGATCTGGAATGTTTAGAC
CATTGGAGAAACGGCCCGTANGACGATGAGTTGGGAAATCGATACCATAAGGGTGGGGAT
TCAATTGTAAATGGCTTCGTGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAAAAAGAGAAATTACCAATTTTTT

Last Updated on 06.12.2018 16:11