Sequences from confirmation amplicon


Confirmation sequences for line 097C10 and hit At1g53240 are shown.
Line ID 097C10
Locus At1g53240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19856232
Primers used for PCR CP03 - 8474, show primer details
   
Sequence Name 06-K031007-022-097-C10C-8409
GenBank Accession LN488686 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K031007-022-097-C10C-8409
GCCATTTACAATTGAATATATACTCATTTAGATCATATGCTTACATGTGGGCAGTACTTG
GCGATGGCAGTGCAAAGGTTCTTGACAATTCCAGCATTAATGTTGAAAAGATCGTCACGG
GTCATACCAGGCTTCCTTGGTACACCAGCTGGAATGATAACGAGATCAGCTCCTTCAAGA
GCTTTGGCCAAGTTATCATCGCCCATGTATCCAACAACCTATCAAACACAAGTACATAGC
ACATGAATCAGTATAGTGAACAAGTCACAAGTCTAATAAGCCAAAATGATACATCTCTGT
TCAAAATCTGAACGAGGAAGCAATTCAAATCAAAAAGGGATTTTGTTTGTAATACCTCAG
ATCTGGTGTTGATGTGACCAACATCAGCAGCAACTCCTGGAGTGTTAGCGATATCGTAGA
GGG
   
Sequence Name 07-K031007-022-097-C10C-CP03
GenBank Accession LN489148 [GenBank]
Sequencing primer gene specific primer, CP03: 5'-CTCCCTCTACGATATCGCTAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K031007-022-097-C10C-CP03
CATCAACACCAGAATCTGAAGGTATTACAAACAAAATCCCTTTTTGATTTGAATTGCTTC
CTCGTTCAGATTTTGAACAGAGATGTATCATTTTGGCTTATTAGACTTGTGACTTGTTCA
CTATACTGATTCATGTGCTATGTACTTGTGTTTGATAGGTTGTTGGATACATGGGCGATG
ATAACTTGGCCAAAGCTCTTGAAGGAGCTGATCTCGTTATCATTCCAGCTGGTGTACCAA
GGAAGCCTGGTATGACCCGTGACGATCTTTTCAACATTAATGCTGGAATTGTCAAGAACC
TTTGCACTGCCATCGCCAAGTACTGCCCACATGTAAGCATATGATCTAAATGAGTATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTACATTCAC

Last Updated on 06.12.2018 16:11