Sequences from confirmation amplicon


Confirmation sequences for line 098C02 and hit At3g03880 are shown.
Line ID 098C02
Locus At3g03880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:998344
Primers used for PCR BH73 - 8474, show primer details
   
Sequence Name 46-K104418-0022-098-C02C-BH73
GenBank Accession LN505857 [GenBank]
Sequencing primer gene specific primer, BH73: 5'-TGGATCACTGGAATGAAATTGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K104418-0022-098-C02C-BH73
TTCCCCTAACATCGTATCTATCAGGGCACAAATCAGCCAACCATGTTCCAGGACTCACCA
ACTGTTTAAAATCAGACAACACATTATTCTCAAAACTATAAACAGACAACTGAAGAATCT
TCAGAACACTTTTCTAAAAACGACAAAAAAGAGTATGGGAAACTAACAAGTAAGCTTCTT
TGGATGAGTTTAGCTCTCTTCTTAGGCCTATGAGAAGGTTTGCAACCTTTCATAGCCATG
AAATCTTCTTCTTTCTCTTTGTTTGATAATGTTATAAAGAGCTTAGGCCACATACTCTCT
TCCTTGTTGTTGCTATCTTCTCCATTGATGTTTCCATCTAAACAATCTTTCCCAATGTTA
TCCACTACACCTCTCGTAGTATAATACCTCTCCTCTTTCTCCGGAGACGGTCTCCGGCGA
TCTGGTAGTCCAGATCTGAGGATTGCTCCTTCTGATCCTCTACAAAAACCCACCAAGAAA
TTATGAGTATATTCAATTGTCATTATTCAATTGTCACCGAAGTAATGTCATTATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
GGTTATT
   
Sequence Name 62-K104419-0022-098-C02C-8409
GenBank Accession LN510752 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K104419-0022-098-C02C-8409
GCCATTTACATTGAATAATGACATTACTTCGGTGACAATTGAATAATGACAATTGAATAT
ACTCATAATTTCTTGGTGGGTTTTTGTAGAGGATCAGAAGGAGCAATCCTCAGATCTGGA
CTACCAGATCGCCGGAGACCGTCTCCGGAGAAAGAGGAGAGGTATTATACTACGAGAGGT
GTAGTGGATAACATTGGGAAAGATTGTTTAGATGGAAACATCAATGGAGAAGATAGCAAC
AACAAGGAAGAGAGTATGTGGCCTAAGCTCTTTATAACATTATCAAACAAAGAGAAAGAA
GAAGATTTCATGGCTATGAAAGGTTGCAAACCTTCTCATAGGCCTAAGAAGAGAGCTAAA
CTCATCCAAAGAAGCTTACTTGTTAGTTTCCCATACTCTTTTTTGTCGTTTTTAGAAAAG
TGTTCTGAAGATTCTTCAGTTGTCTGTTTATAGTTTTGAGAATAATGTGTTGTCTGATTT
TAAACAGTTGGTGAGTCCTGGAACATGGTTGGCTGATTTGTGCCCTGATAGATACGATGT
TAGGGTGAAGAAGAGCTCTAAGAAGGTATGACCTTTTTATCAATTTCATTCAGNTGATCC
Primers used for PCR 52wr - 3144, show primer details
   
Sequence Name 79-K118835-0022-098-C02C-52wr
GenBank Accession LN513883 [GenBank]
Sequencing primer gene specific primer, 52wr: 5'-TTATCCCCAAAAAGAGAGGAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K118835-0022-098-C02C-52wr
CTTAAGACCATTGGCAGTAATTTTTAAAAGGTAATAAAAGTCATTTGCCAGTGGGAAAAA
GGGCTAGACCGTAAATTACCACACTCGGTCTCAAATCAGACCGTCAATTTTATAATCGAA
CGGTTAGTAAAGAACCCAAAGAGATGCCCTAAAGGCATATAAACCACCTTTACCTCCCAT
TTTACAGAGTCGGTAGATAGAGAGAAAGACGCTTCTGGTCAGTTTTCGTTAAACCCGCCG
GAGAAACTAGCGACTACGGTGGTCGAACCCGTCTGACGTCGTAGAATTCATCGTAATCTT
CACTTCCTTTCTTCCCCGACAAAATCATCGATCTGTGTTTGCCAATCAGATTCACCGGAA
CTTCTAGGTAACTTGAGAACAATTTGCAGAGCTCACGCTTCCTTGTCTAATTTCTCTTCA
CATCAATTTGAATCAATCTCTCTTTTGATTTTCAGTTCCTAACATTGAAAATAATCTTCA
CTTTTGGATAAATTTTTCAGTTAGGACGATTGTGAAAAATGTCGTCATCGTTAGAACAAG
AAATCTTCTTACAGTGGGGAAACAAGAAGAGATTGAGATGTCTTAGAGCCAAAGCCAAAA
AGATCTCTCGTTCTAAAAACTCTTCTCGCTTTCTAGGTCAAGATACCGTCCTCCTTCAAT
CCTCTAGATTCTCCAGGTTAACTTTGTGATTCCTTTATTTACTGTTGTGAAATTGCTCAA
TTCCACTCTTGGCATCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCT
CCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTG
CGTCATCCCTTACGTCAGTGGAGAT
   
Sequence Name 55-K118834-0022-098-C02C-35St
GenBank Accession LN508951 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118834-0022-098-C02C-35St
TCCTTGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGATGCCAAGAGTGGAATTGAGCAATTTCACA
ACAGTAAATAAAGGAATCACAAAGTTAACCTGGAGAATCTAGAGGATTGAAGGAGGACGG
TATCTTGACCTAGAAAGCGAGAAGAGTTTTTAGAACGAGAGATCTTTTTGGCTTTGGCTC
TAAGACATCTCAATCTCTTCTTGTTTCCCCACTGTAAGAAGATTTCTTGTTCTAACGATG
ACGACATTTTTCACAATCGTCCTAACTGAAAAATTTATCCAAAAGTGAAGATTATTTTCA
ATGTTAGGAACTGAAAATCAAAAGAGAGATTGATTCAAATTGATGTGAAGAGAAATTAGA
CAAGGAAGCGTGAGCTCTGCAAATTGTTCTCAAGTTACCTAGAAGTTCCGGTGAATCTGA
TTGGCAAACACAGATCGATGATTTTGTCGGGGAAGAAAGGAAGTGAAGATTACGATGAAT
TCTACGACGTCAGACGGGTTCGACCACCGTAGTCGCTAGTTTCTCCGGCGGGTTTAACGA
AAACTGACCAGAAGCGTCTTTCTCTCTATCTACCGACTCTGTAAAATGGGAGGTAAAGGT
GGTTTATATGCCTTTAGGGCATCTCTTTGGGTTCTTTACTAACCGTTCGATTATAAAATT
GACGGTCTGATTTGAGACCGAGTGTGGTAATTTACGGTCTAGCCCTTTTCCCACTGGCAA
ATGACTTTTATTACCTTTTAAAAATTACTGCCCATGGTCTTTAAGGGAACATAGCTTGAA
AATGGAGCT

Last Updated on 06.12.2018 16:11