Sequences from confirmation amplicon


Confirmation sequences for line 098G07 and hit At3g10270 are shown.
Line ID 098G07
Locus At3g10270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3176621
Primers used for PCR 11ra - 8474, show primer details
   
Sequence Name 17-K116083-0022-098-G07C-11ra
GenBank Accession LN494003 [GenBank]
Sequencing primer gene specific primer, 11ra: 5'-TCAAGAGGTTCATATTGAATGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116083-0022-098-G07C-11ra
ATGATCCGAAAGGGACGTGTATAGTGAATATTTTTATGCTGGAGGGTTAACTGAATATGT
TAGCTGGCTAAATACTGATAAGGTGAGGCATGTACTGTGGGTCCCTTGTTATAGTTGTAT
AAAATCTCAATATTTGCCAATCGGTGATGCTAGACTTAAAATAGTGGCAATTAAATTGGA
ATGGGTTTTGTGAAACAAAATGCAATGGGGATGGTACTAATACTTTTCCTTCTCAGCTTA
CTTGATGGTAAGATGTGTTGTCTGTTCATTATTCTTTAGGGCTCTATTTAGCATGAGCAT
GGAACCTTTGTAAACTTAATCTCTATTATGTTAGCTCTTATGCATTTAGGTCTCTGGTGT
TATAGGTCATCCTTATTACTTTTCCCTTCCGAAAATATGAATTTACAATTCGTTTTGTTC
AGAAACCGCTTCATGATGTGCTGGGTTTCCGGAAAGAGATAAATGGCTCCACCGTTGACG
TTTCACTTCAGTGGTATTTTACTCTTCCTTCTACAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 01-K116082-0022-098-G07C-8409
GenBank Accession LN486719 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116082-0022-098-G07C-8409
TCATTGAATATATCCTGTAGAAGGAAGAGTNAATACCACTGAACTGTAACGTCAACGGTG
GAGCCATTTATCTCTTTCCGGAAACCCAGCACATCATGAAGCGGTTTCTGAACAAAACGA
ATTGTAAATTCATATTTTCGGAAGGGAAAAGTAATAAGGATGACCTATAACACCAGAGAC
CTAAATGCATAAGAGCTAACATAATAGAGATTAAGTTTACAAAGGTTCCATGCTCATGCT
AAATAGAGCCCTAAAGAATAATGAACAGACAACACATCTTACCATCAAGTAAGCTGAGAA
GGAAAAGTATTAGTACCATCCCCATTGCATTTTGTTTCACAAAACCCATTCCAATTTAAT
TGCCACTATTTTAAGTCTAGCATCACCGATTGGCAAATATTGAGATTTTATACAACTATA
ACAAGGGACCCACAGTACATGCCTCACCTTATCAGTATTTAGCCAGCTAACATATTCAGT
TAACCCTCCAGCATAAAAATATTCACTATACACGTCCCTTTCTGGATCATCATCCTCCTT
TTTTAGAGAGATTGTAACCTGCATTCAATATGAACCCTCTTG

Last Updated on 06.12.2018 16:11