Sequences from confirmation amplicon


Confirmation sequences for line 099A02 and hit At4g14096 are shown.
Line ID 099A02
Locus At4g14096 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8125476
Primers used for PCR PC38 - 8474, show primer details
   
Sequence Name 39-K103787-0022-099-A02C-PC38
GenBank Accession LN503328 [GenBank]
Sequencing primer gene specific primer, PC38: 5'-TTGAAAAATTGAGTCAACAAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103787-0022-099-A02C-PC38
AGATTTGGAGAATTCTTGAGCAAACCTGGCAATGACTGCCATCCTACTTCGGGATTACTC
TCGATGGTTAAATGAGTCAGGTTGTTGAATATTGGTATAGCATCACAACTGTAAGTAAGT
ACCTGAAACACAACCACAACAACTAGATTATCATCACTCAATTGCAATCACTAACTCAAA
TCCTTGAACAATGTTAAAAAGATTACAAGTTGTTTTTCATGATGAAAACAAACCTGAAGA
GTGTTGGAAGATAGATAGAGAGTCTTCACATTTGCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 55-K103788-0022-099-A02C-8409
GenBank Accession LN508789 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103788-0022-099-A02C-8409
AAGACTCTCTATCCATCTTCCAACACTCTTCAGGGTTTGTTTTCATCATGAAAAACAACT
TGTAATCTTTTTAACATTGTTCAAGGATTTGAGTTAGTGATTGCAATTGAGTGATGATAA
TCTAGTTGTTGTGGTTGTGTTTCAGGTACTTACTTACAGTTGTGATGCTATACCAATATT
CAACAACCTGACTCATTTAACCATCGAGAGTAATCCCGAAGTAGGATGGCAGTCATTGCC
AGGTTTGCTCAAGAATTCTCCAAATCTTGAAACTCTTATCTTCCAAGTAAGAAACAAAAC
CCTTGGAAAGCATTTGTTGACTCAATTTTTCA

Last Updated on 06.12.2018 16:11