Sequences from confirmation amplicon


Confirmation sequences for line 099D10 and hit At1g60190 are shown.
Line ID 099D10
Locus At1g60190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22199592
Primers used for PCR BB72 - 8474, show primer details
   
Sequence Name 02-K104123-0022-099-D10C-BB72
GenBank Accession LN487008 [GenBank]
Sequencing primer gene specific primer, BB72: 5'-ATCACCGTAATCGCAACTTTTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104123-0022-099-D10C-BB72
GGAATCGCATCAGAGATTTCTCCGATCAATCTGCTGTAATCTCCGAGAGAAGATAGATAA
AACAGAGCAGCTGCTGCGTATTGTCTGCTCTCTCTTCTCGCACCATCGTTTAAAACCTCA
ACGATTAGCCTTAATCCACCACCGTCTTCTCCGACGATTCGAGTTTTTCCGGCGATATCT
TTCGATAAATTCATAATCCCAGCCATGGCGTTCTCTTGGATCCTCGGATCATCCGAACGA
AGAATCTTCATCAACGATTCAACAACTCCAGCTTCCACTAAACACGATCTGTAAAAAGTA
CTCGTCTTGGTGAGAATTCGAATCTACATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAAATGTCCGCACGGTT
   
Sequence Name 18-K104124-0022-099-D10C-8409
GenBank Accession LN494280 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K104124-0022-099-D10C-8409
AAGCCATGTAGATTCGAATTCTCACCAAGACGAGTACTTTTTACAGATCGTGTTTAGTGG
AAGCTGGAGTTGTTGAATCGTTGATGAAGATTCTTCGTTCGGATGATCCGAGGATCCAAG
AGAACGCCATGGCTGGGATTATGAATTTATCGAAAGATATCGCCGGAAAAACTCGAATCG
TCGGAGAAGACGGTGGTGGATTAAGGCTAATCGTTGAGGTTTTAAACGATGGTGCGAGAA
GAGAGAGCAGACAATACGCAGCAGCTGCTCTGTTTTATCTATCTTCTCTCGGAGATTACA
GCAGATTGATCGGAGAAATCTCTGATGCGATTCCGGGATTGGTGAGAATCGTTAAAAGTT
GCGATAACGGTGAT

Last Updated on 06.12.2018 16:11