Sequences from confirmation amplicon


Confirmation sequences for line 099H04 and hit At2g18960 are shown.
Line ID 099H04
Locus At2g18960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8223223
Primers used for PCR 66pm - 8474, show primer details
   
Sequence Name 79-K115263-0022-099-H04C-66pm
GenBank Accession LN513863 [GenBank]
Sequencing primer gene specific primer, 66pm: 5'-CTGAAAACAGAGAAACAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K115263-0022-099-H04C-66pm
AGCATCCACCAACATATTACCTTGGTTTTAGGAGCAAGACCAGCCATGAGAGCAGCTGCA
GCATTTCCGGCGTTGTTTTCTTCAATGAAACTGATTGTGGAGTTGATAACAAGCAGACAG
ATAATACCCACAAAATCTTGCCAATCCGGAGGTCGATTATCACCATTAGCCAAAGCAATG
GCCATGAGAGCTGCAGCTTCCATAACCCATGAAAGCGGATTCCACATGAACCCCAGAAAC
TTCAGAATTTTGCTTTCCTGATATTTTTTTACGAAAGATCAACCTCGTGAGTTAAACAAA
ACCAAAGCTTTCAATGATGATAAAACAACAATGCTAAGTCAAACTCAAACCTTCTTCTCT
TCGAGCTTGTTGGGGCCAAATATCACAATCCTGTCTTCCCCTTCCTGCGTTGTCAATCCT
TCCCTTGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATATCCGCA
   
Sequence Name 91-K115262-0022-099-H04C-8409
GenBank Accession LN515135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K115262-0022-099-H04C-8409
CATTTCATTGAATATACAGGGAAGGATTGACAACGCAGGAAGGGGAAGACAGGATTGTGA
TATTTGGCCCCAACAAGCTCGAAGAGAAGAAGGTTTGAGTTTGACTTAGCATTGTTGTTT
TATCATCATTGAAAGCTTTGGTTTTGTTTAACTCACGAGGTTGATCTTTCGTAAAAAAAT
ATCAGGAAAGCAAAATTCTGAAGTTTCTGGGGTTCATGTGGAATCCGCTTTCATGGGTTA
TGGAAGCTGCAGCTCTCATGGCCATTGCTTTGGCTAATGGTGATAATCGACCTCCGGATT
GGCAAGATTTTGTGGGTATTATCTGTCTGCTTGTTATCAACTCCACAATCAGTTTCATTG
AAGAAAACAACGCCGGAAATGCTGCAGCTGCTCTCATGGCTGGTCTTGCTCCTAAAACCA
AGGTAATATGTTGGTGGATGCTGTGACATTACTGTACAAGATATTTCGAGACTTCTAGAG
AGCTTAACCTGTTTCCTGNTTTTCA

Last Updated on 06.12.2018 16:11