Sequences from confirmation amplicon


Confirmation sequences for line 100G09 and hit At4g21910 are shown.
Line ID 100G09
Locus At4g21910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11626990
Primers used for PCR PW18 - 8474, show primer details
   
Sequence Name 05-K024844-022-100-G09C-8409
GenBank Accession LN488192 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K024844-022-100-G09C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCTCAGCTGCTGATCCATG
TAGATTTTATTCGCAGATTCTTGTTCTTCTTGCTGGTTTGCTCGAAAATCCTGCTCGCTC
TCTAGATTCTCTTTCCATCTGGTAAGTCCATTAATTAGTTAGTAACTTGATTCTTGATTC
TATATCATTTTCTTAATCACTTATACTTTTTTTTTTTGGTTTTAATGTGAAGGAATCACT
TATACTTCTCTTTTATCTAACTATTTTTTTCTTGTTATTTTTGGGCAGTATGTCAATTTC
AGCATTATCATTCAGGGTCTCTGTCGGCTTTAATGCTGCTGTAAGGTTAGTATTTGTTTC
TCAATCAATTTCAAATTATCATCCAAACATTGCTCAGGTTAAATCATAATTTTCTATACA
ATTACTATGCATGGACAGTGTACGGACAAGTAATGAGCTTGGAGCAGGAAATCCGAAATC
AGCATGGTTCTCTACATGGACGGCGACTTTTGTATCCTTCGGGATCTCCGTGACGGAAGC
CCTCGCCGTGATTTGGTT
   
Sequence Name 06-K024844-022-100-G09C-PW18
GenBank Accession LN488642 [GenBank]
Sequencing primer gene specific primer, PW18: 5'-TAACGTAATCACGAAACCAAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K024844-022-100-G09C-PW18
ACGGCGAGGGCTTCCGTCACGGAGATCACGAAGGATACAAAAGTCGCCGTCCATGTAGAG
AACCATGCTGATTTCGGATTTCCTGCTCCAAGCTCATTACTTGTCCGTACACTGTCCATG
CATAGTAATTGTATAGAAAATTATGATTTAACCTGAGCAATGTTTGGATGATAATCTGAA
ATTGATTGAGAAACAAATACTAACCTTACAGCAGCATTAAAGCCGACAGAGACCATGAAT
GATAATGCTGAAATTGACATACTGCACAAAAATAACAAGAAAAAAATAGTTAGATAAAAG
AGAAGTATAAGTGATTCCTTCACATTAAAACCAAAAAAAAAAAGTATAAGTGATTAAAAA
AATGATATAGAATCAAGAATCAAGTTACTAACTAATTAATGGACTTACCAGATGGAAAGA
GAATCTAGAGAGCGAGCAGGATTTTCGAGCAAACCAGCAAGAAGAACAAGAATCTGCGAA
TAAAATCTACATGGATCAGCAGCTGAGCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT

Last Updated on 06.12.2018 16:11