Sequences from confirmation amplicon


Confirmation sequences for line 100H05 and hit At3g56350 are shown.
Line ID 100H05
Locus At3g56350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20894548
Primers used for PCR CD41 - 8474, show primer details
   
Sequence Name 02-K104268-0022-100-H05C-CD41
GenBank Accession LN487014 [GenBank]
Sequencing primer gene specific primer, CD41: 5'-CATCTTCGGTACTGCCGTATTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104268-0022-100-H05C-CD41
AAACAAAGCACTACGACATATAAAACTCAGTTAATATCAAGATCACGAGTGTGCTTCTCG
AAGACGTCCGCTGCATATTTCCAGTTGATTACAGTCCATATGTTCTTCAAGTACTCCGCT
CTTGCATTCTTGTACTGCATTACCCGCACCCATTGATCAATGTTAATATTCAATCAAACA
GTTCTTTTACTAATAAAACCGATATTACTCTACCTGATATCAAATCAAGGAGTATTATAA
TTGAGATTAGAGTACCTGTGGATAGTAGGCATGCTCCCACACGTCAATCCCAATTAGAGG
AACTAGGTGTGATCCCTTAGTCACCAATGGATCCTGTATGTTCGACCAATCTATCANATC
TTTTTCTTTTCTTTTTCCCAAATCCTTGTGAGATAGACCATGTTACAAATTAGCTTAATC
ACTACATTAATAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTA
   
Sequence Name 18-K104269-0022-100-H05C-8409
GenBank Accession LN494286 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K104269-0022-100-H05C-8409
GATTAGCTAATTTGTACATGGTCTATCTCACAAGGATTTGGGAAAAAGAAAAGAAAAAGA
TTTGATAGATTGGTCGAACATACAGGATCCATTGGTGACTAAGGGATCACACCTAGTTCC
TCTAATTGGGATTGACGTGTGGGAGCATGCCTACTATCCACAGGTACTCTAATCTCAATT
ATAATACTCCTTGATTTGATATCAGGTAGAGTAATATCGGTTTTATTAGTAAAAGAACTG
TTTGATTGAATATTAACATTGATCAATGGGTGCGGGTAATGCAGTACAAGAATGCAAGAG
CGGAGTACTTGAAGAACATATGGACTGTAATCAACTGGAAATATGCAGCGGACGTCTTCG
AGAAGCACACTCGTGATCTTGATATTAACTGAGTTTTATATGTCTGTAGTAGCTTTTGTT
TTTTGGGTTTCTTGTCTTGTTGATAAATACGGCAGTACCGAAGATG

Last Updated on 06.12.2018 16:11