Sequences from confirmation amplicon


Confirmation sequences for line 100H11 and hit At5g03350 are shown.
Line ID 100H11
Locus At5g03350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:816130
Primers used for PCR GA10 - 8474, show primer details
   
Sequence Name 80-K100900-0022-100-H11C-8409
GenBank Accession LN513916 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K100900-0022-100-H11C-8409
CATTTACATTGAATCCCGACATGGTTATCATTAATGTCTCCAAACCTTTGTCCTGAAAAA
CGTCGAATTCGACAGCAAAGATGTGGTTTTTGGGGTTGCCATTGTTGGTTTTGTTGAATA
TCCCGAGGTATCCAGCGGGTCCAGGACCGTCACTTTCAATGGAAGGGACGACAACAAAAG
CAAGGCCGTGACCAGGGGCGGGTTTGATGCGAGTAGAGATGGAGAAAGTGAAAGAAGTTT
CAAATGGATAAGGAGAAGAAGAGTTTGAAGAAGGCTTGAAAGGGACTGGAGTGGACCAAA
GACTTTGGCCGTGAGAGAATGGGTTCTCGTCTCGTGTCATGGAGAGAGCTCCGGAGCGGC
TGACGCCATCAGAAGCAGGACCGAGCTCAGCGTCTCCAAGGAAGACCAACTCTGAGGTTT
TGAGGTTGAGGTTGAGGTTGACGGCGGAAGTGGTGTGAGCTAAGAGCAGAGCAAGAAAAC
AGAGTTTATGAATCTTCATTGCGTTTTCGTAACTGCTGTTTACTCAAGTGAGAGTTTGAA
AGAGTGAAATTATTAACGTGGTGATTTATAAAGAGTAAAACATAAGCCCATGTACATGAC
GTCATATTGTATGATGTCAAGTAAACG
   
Sequence Name 80-K100906-0022-100-H11C-GA10
GenBank Accession LN513917 [GenBank]
Sequencing primer gene specific primer, GA10: 5'-TTGTAGGTGATGCGTTTACTTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K100906-0022-100-H11C-GA10
GGGCTATGTTTACTCTTTATAAATCACCACGTTAATAATTTCACTCTTTCAAACTCTCAC
TTGAGTAAACAGCAGTTACGAAAACGCAATGAAGATTCATAAACTCTGTTTTCTTGCTCT
GCTCTTAGCTCACACCACTTCCGCCGTCAACCTCAACCTCAACCTCAAAACCTCAGAGTT
GGTCTTCCTTGGAGACGCTGAGCTCGGTCCTGCTTCTGATGGCGTCAGCCGCTCCGGAGC
TCTCTCCATGACACGAGACGAGAACCCATTCTCTCACGGCCAAAGTCTTTGGTCCACTCC
AGTCCCTTTCAAGCCTTCTTCAAACTCTTCTTCTCCTTATCCATTTGAAACTTCTTTCAC
TTTCTCCATCTCTACTCGCATCAAACCCGCCCCTGGTCACGGCCTTGCTTTTGTTGTCGT
CCCTTCCATTGAAAGTGACGGTCCTGGACCCGCTGGATACCTCGGGATATTCAACAAAAC
CAACAATGGCAACCCCAAAAACCACATCTTTGCTGTCGAATTCGACGTTNTTCAGGACAA
AGGTTTTGGAGACATTAATGATAACCATGTCGGGATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCANAAATGTAGATT
Primers used for PCR GA11 - 3144, show primer details
   
Sequence Name 81-K100901-0022-100-H11C-3144
GenBank Accession LN514033 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K100901-0022-100-H11C-3144
ATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTACAT
GAAGACATGAAGCCATTTCTGTTACTTCCGTTGTTGCTGAGAAAGCTGGTTATTGGGTTC
AGACAGGAATTGGGAAAATGAAACACTGGTCGTTTAAAGAGTTCAAGCTGAGTAATGGAG
AGAGGTACAAGGCTTGGATTGAGTATAGAAACTCCAAGGTTACAGTTACTCTCGCGCCAG
AAACCGTTAAGAAACCTAAGAAGCCTTTGATCGTTGCCCATTTAGATCTCTCTAAGGTTT
TTCTTCAAAATATGTACCCCGGTTTTTCTGGTGCCATGGGCCGTGGCGTTGAACGTCATG
ACATTTGGAGTTGGACCTTCCAGAACAGTGCCAAGAGAATCTAAACCGGTTCTGTTCGGT
TATGCTTTTTCTAATGTATCAGAAAGTTGTAACCGGAATGAATAAAATAGCAAATTTTGT
TGTTTTAATTGTTTGTATTTGACTATTTACACAGTATCCTATCCTGCTAGGACCTGTGCA
CTTGTTGTTATGTTGATTCTTCATTCTAAAAGATTCTAAGTAGAGACCTGACAC
   
Sequence Name 81-K100907-0022-100-H11C-GA11
GenBank Accession LN514034 [GenBank]
Sequencing primer gene specific primer, GA11: 5'-GTGATGTTTCTGTGTGTCAGGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K100907-0022-100-H11C-GA11
GATCACATAACAACAAGTGCACAGGTCCTAGCAGGATAGGATACTGTGTAAATAGTCAAA
TACAAACAATTAAAACAACAAAATTTGCTATTTTATTCATTCCGGTTACAACTTTCTGAT
ACATTAGAAAAAGCATAACCGAACAGAACCGGTTTAGATTCTCTTGGCACTGTTCTGGAA
GGTCCAACTCCAAATGTCATGACGTTCAACGCCACGGCCCATGGCACCAGAAAAACCGGG
GTACATATTTTGAAGAAAAACCTTAGAGAGATCTAAATGGGCAACGATCAAAGGCTTCTT
AGGTTTCTTAACGGTTTCTGGCGCGAGAGTAACTGTAACCTTGGAGTTTCTATACTCAAT
CCAAGCCTTGTACCTCTCTCCATTACTCAGCTTGAACTCTTTAAACGACCAGTGTTTCAT
TTTCCCAATTCCTGTCTGAACCCAATAACCAGCTTTCTCAGCAACAACGGAAGTAACAGA
AATGGCTTCATGTCTTCATGTACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCNAATGAAATGAACTTCCTTATATAGAGGAAGGGTC
TTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT

Last Updated on 06.12.2018 16:11