Sequences from confirmation amplicon


Confirmation sequences for line 101C11 and hit At5g38450 are shown.
Line ID 101C11
Locus At5g38450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15396874
Primers used for PCR MS67 - 8474, show primer details
   
Sequence Name 23-K017400-022-101-C11C-MS67
GenBank Accession LN496352 [GenBank]
Sequencing primer gene specific primer, MS67: 5'-GAATTAAATGGAAACGTTTAGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K017400-022-101-C11C-ms67
AAAATATCCAGATTTCTAATTTAATTCGACTCAATATCTTCCATTTCTTTAAGTGAGAAT
ATGCAACAAAGTTATACCATAATTAGTTCTCCCAGATTTAGTACCCTACATGAATTTATA
TTGTATCTCGACACTATAAATACACCATTACTCTTTATCTCACCCTCTTAATATCTCTTG
TTTTTCTTTTCGAATCTTTGTTGCTGGAAGAAGAAAAGAAAAAACCCAAAAATGTTGCTT
ACTATATTAAAATCACTCCTCGTGATATTCGTGACTACGATATTGAGAGTTTTATACGAC
ACCATATCGTGCTACTGGCTAACACCTAGACGAATCAAGAAGATCATGGAACAGCAAGGC
GTAACGGGTCCTAAACCGCGTCCGTTAACCGGAAATATCCTTGAAATCTCGGCCATGGTT
TCGCAATCCGCTTCCAAGGATTGTGACTCTATTCACCATGACATCGTCGGCCGCCTTCTC
CCGCATTACGTTGCTTGGTCCAAACAATACGGTATGGTCTTGAATTGTAACAAGTTTGTG
TTTTCTTGGTCGTGAAGTCTATACATAGATTTCGTTATTAAGTTGGTCTGTCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTTATTATAAAAT
   
Sequence Name 24-K017400-022-101-C11C-8409
GenBank Accession LN496793 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K017400-022-101-C11C-8409
AGATTTTCCGGACATGAAGCCATTTACAATTGAATATATCCTGACAGACCAACTTAATAA
CGAAATCTATGTATAGACTTCACGACCAAGAAAACACAAACTTGTTACAATTCAAGACCA
TACCGTATTGTTTGGACCAAGCAACGTAATGCGGGAGAAGGCGGCCGACGATGTCATGGT
GAATAGAGTCACAATCCTTGGAAGCGGATTGCGAAACCATGGCCGAGATTTCAAGGATAT
TTCCGGTTAACGGACGCGGTTTAGGACCCGTTACGCCTTGCTGTTCCATGATCTTCTTGA
TTCGTCTAGGTGTTAGCCAGTAGCACGATATGGTGTCGTATAAAACTCTCAATATCGTAG
TCACGAATATCACGAGGAGTGATTTTAATATAGTAAGCAACATTTTTGGGTTTTTTCTTT
TCTTCTTCCAGCAACAAAGATTCGAAAAGAAAAACAAGAGATATTAAGAGGGTGAGATAA
AGAGTAATGGTGTATTTATAGTGTCGAGATACAATATAAATTCATGTAGGGTACTAAATC
TGGGAGAACTAATTATGGTATAACTTTGTTGCATATTCTCACTTAAAGAAATGGAAGATA
TTGAGACTAATTAAATTAGTAAATCTCGTTATTTTTTACCTTGCTTGGCTAAACGTT

Last Updated on 06.12.2018 16:11