Sequences from confirmation amplicon


Confirmation sequences for line 101D04 and hit At5g63120 are shown.
Line ID 101D04
Locus At5g63120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25321607
Primers used for PCR UF25 - 8474, show primer details
   
Sequence Name 13-K030219-022-101-D04C-8409
GenBank Accession LN491912 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K030219-022-101-D04C-8409
AGCCATTTACAATTGAATATATTCAGCAATCTCTATGTGGAGAGTCCCACTGTGCAAGCT
ATGACAGAGCAGGATGTTGCAATGTACAGAACCGAGAGGGATATATCCGTTGAAGGTCGT
GATGTTCCTAAGCCGATGAAGATGTTCCAAGATGCTAACTTTCCAGGTATTTTGATTGGA
TTGGTCATAGAGTTTCCAGTTCATTTATATCTAATTTGGGTTCTTAGTTTAATGGTTCCT
TTTATCTGTTAGATAACATTCTTGAAGCAATTGCTAAATTGGGATTTACTGAGCCAACAC
CTATACAAGCTCAGGGATGGCCGATGGCGTTGAAGGGTAGGGATTTGATTGGTATTGCTG
AGACTGGCTCTGGTAAGACATTGGCTTACTTGTTACCAGCATTGGTTCACGTTAGTGCAC
AACCTCGATTAGGTGAGTATTACAGCACTATGTGATATTCTGATTTAAGCTGCATTCATT
TTTCCGGCAACTTTTTTATTTGCATTGTTGGGGTGTAATATAGGTCAGGATGATGGCCCC
ATTGTGTTAATATTAGCTCCCACAAGAGAACTAGCTGTTCAGATTCAAGAGGAATCAAGG
AAATTCGGGTTGAGGTCTGGTGTTAGAAGTACTTGTATCTATGGTGGTGCTCCTAAAGGA
CCTC
   
Sequence Name 14-K030219-022-101-D04C-UF25
GenBank Accession LN492383 [GenBank]
Sequencing primer gene specific primer, UF25: 5'-GATCACGAATCTGAGGTCCTTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K030219-022-101-D04C-UF25
TTCTACACCAGACCTCAACCCGAATTTCCTTGATTCCTCTTGAATCTGAACAGCTAGTTC
TCTTGTGGGAGCTAATATTAACACAATGGGGCCATCATCCTGACCTATATTACACCCCAA
CAATGCAAATAAAAAAGTTGCCGGAAAAATGAATGCAGCTTAAATCAGAATATCACATAG
TGCTGTAATACTCACCTAATCGAGGTTGTGCACTAACGTGAACCAATGCTGGTAACAAGT
AAGCCAATGTCTTACCAGAGCCAGTCTCAGCAATACCAATCAAATCCCTACCCTTCAACG
CCATCGGCCATCCCTGAGCTTGTATAGGTGTTGGCTCAGTAAATCCCAATTTAGCAATTG
CTTCAAGAATGTTATCTAACAGATAAAAGGAACCATTAAACTAAGAACCCAAATTAGATA
TAAATGAACTGGAAACTCTATGACCAATCCAATCAAAATACCTGGAAAGTTAGCATCTTG
GAACATCTTCATCGGCTTAGGAACATCACGACCTTCAACGGATATATCCCTCTCGGTTCT
GTACATTGCAACATCCTGCTCTGTCATAGCTTGCACAGTGGGACTCTCCACATAGAGATT
GCTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11