Sequences from confirmation amplicon


Confirmation sequences for line 101F07 and hit At2g25170 are shown.
Line ID 101F07
Locus At2g25170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10723631
Primers used for PCR FV33 - 3144, show primer details
   
Sequence Name 77-K033880-022-101-F07C-FV33
GenBank Accession LN513492 [GenBank]
Sequencing primer gene specific primer, FV33: 5'-TGAGCTTTATTGAGGTTGATGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K033880-022-101-F07C-FV33
TCCTAAGACTGATCCTATCAGTAAGTTCCATCACAAGTTTCTTTATTTAACGAGTTGTTG
ATTCTAATGTGAGCTCTCTGAATCTCGCTGCAGCTTCAGAAGAAATTATGGGGGCTGCTG
TTGACAACAACCAAGCGCGGGTCGAAATAGCTCAACATTATAACCAGGTAAGCTATGCTT
TTTTCCTTTGGTGGTAGGCTAATGTCTAGAACTAGTATATCACACTAATATCTCTCCGGT
TATTCAGATGTGCAAACTTCTTGATGAGAACGCTCGGGAATCAGTCCAAGCATATGTAAA
CAACCAACCACCGAGTACCAAGGTGAATGAGAGCTTCCGTGCACTCAAATCTATCAATGG
TAACATTAACACAATCCTTTCGATTACATCTGATCAATCCAAGTCACATGAAGACGACAC
ATAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTG
TCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGG
ATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACG
TGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG
   
Sequence Name 21-K033880-022-101-F07C-3144
GenBank Accession LN495573 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K033880-022-101-F07C-3144
ATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTNGGAGA
GGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTT
TATGTGTCGTCTTCATGTGACTTGGATTGATCAGATGTAATCGAAAGGATTGTGTTAATG
TTACCATTGATAGATTTGAGTGCACGGAAGCTCTCATTCACCTTGGTACTCGGTGGTTGG
TTGTTTACATATGCTTGGACTGATTCCCGAGCGTTCTCATCAAGAAGTTTGCACATCTGA
ATAACCGGAGAGATATTAGTGTGATATACTAGTTCTAGACATTAGCCTACCACCAAAGGA
AAAAAGCATAGCTTACCTGGTTATAATGTTGAGCTATTTCGACCCGCGCTTGGTTGTTGT
CAACAGCAGCCCCCATAATTTCTTCTGAAGCTGCAGCGAGATTCAGAGAGCTCACATTAG
AATCAACAACTCGTTAAATAAAGAAACTTGTGATGGAACTTACTGATAGGATCAGTCTTA
GGAAGTCCATCAAGCATTTCATCATCAACCTCATAAAAGCTC
Primers used for PCR FB08 - 8474, show primer details
   
Sequence Name 87-K034733-022-101-F09C-8409
GenBank Accession LN514719 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K034733-022-101-F09C-8409
GCCATTTACATGCATGCATGTCGACTCTAGAGGATCCCCAATTCCCATGGAGTCAAAGAT
TCAAATAGAGGACCTAACAGAACTCGCCGTAAAGACTGGCGAACAGTTCATACAGAGTCT
CTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACGCTTGT
CTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTCA
ACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTAT
TGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAA
GGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAG
GAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGA
TATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTC
TATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTT
TCCCGCCTTCGGTTTAAACTATCAGTGTTAAGAGGTGGCGTCGTCGATCTGAATGTGGTG
GAGGGAGAGGAGAACATTGCTGAAGCTAGTGGAAGTGTTGATGTAAAAATGGAAGAAGCC
AAAGAAGAAGAGAAGCCAAAGAACATGGTCGTTGATTGACTCAACTGGTAAATCAAGATT
CAAGACCCAAGGGGTCATGAAGTAGGAACTGTTGTATTATCTTTGGTTACATGTATGAGA
CTTATTCTTCCTTTTGTAAGACCTATCTTCTGCTTATGCAAACATTGGTAACATTTTAAG
CTAACTTTGATATTCTTCATGTTTTCCTATTTTTGGAAAATCATGAAATTATAGTTTCTC
AATCAAAGCTGTTTGGTAATGCACCTCAAGCTTT
   
Sequence Name 88-K034733-022-101-F09C-FB08
GenBank Accession LN514823 [GenBank]
Sequencing primer gene specific primer, FB08: 5'-AAAGATTCCTTCGTACACTCGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K034733-022-101-F09C-FB08
ACCCAATTTGTATTATAACATTCTTGTGTTTGTATCTTACATTATTTAGTTCAGTTTTTG
AGTTTGATTGAGAATAGTAGTTAGATTGAAACAGACGACGTCTAACCGGAAACATAACCG
GACTTCATCGAACCTATTCACACACTGTTAAAAAGCTTGAGGTGCATAACCAAACAGCTT
TGATTGAGAAACTATAATTTCATGATTTTCCAAAAATAGGAAAACATGAAGAATATCAAA
GTTAGCCTAAAATGTTACCAATGTTTGCATAAGCAGAAGATAGGTCTTACAAAAGGAAGA
ATAAGTCTCATACATGTAACCAAAGATAATACAACAGTTCCTACTTCATGACCCCTTGGG
TCTTGAATCTTGATTTACCAGTTGAGTCAATCAACGACCATGTTCTTTGGCTTCTCTTCT
TCTTTGGCTTCTTCCATTTTTACATCAACACTTCCACTAGCTTCAGCAATGTTCTCCTCT
CCCTCCACCACATTCAGATCGACGACGCCACCTCTTAACACTGATAGTTTAAACCGAAGG
CGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTT
ATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
AGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGA
TGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGGCATCTTCAACGA
TGGCCTTTCCTTTATCGCAATGATGGCATTTGTAGGAGCCACCTTCCTTTTCCACTATCT
TCACAATAAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCGGATATTACCC
TTTGTTGAAAAGTCTCAATTGCCCTTTGGTCT
Primers used for PCR 20em - 3144, show primer details
   
Sequence Name 84-K119577-0022-101-F07C-20em
GenBank Accession LN514483 [GenBank]
Sequencing primer gene specific primer, 20em: 5'-AAAGATTCCTTCGTACACTCGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K119577-0022-101-F07C-20em
CCAATTTGTATATACATTCTTGTGTTTGTATCTTACATTATTTAGTTCAGTTTTTGAGTT
TGATTGAGAATAGTAGTTAGATTGAAACAGACGACGTCTAACCGGAAACATAACCGGACT
TCATCGAACCTATTCACACACTGTTAAAAAGCTTGAGGTGCATAACCAAACAGCTTTGAT
TGAGAAACTATAATTTCATGATTTTCCAAAAATAGGAAAACATGAAGAATATCAAAGTTA
GCCTAAAATGTTACCAATGTTTGCATAAGCAGAAGATAGGTCTTACAAAAGGAAGAATAA
GTCTCATACATGTAACCAAAGATAATACAACAGTTCCTACTTCATGACCCCTTGGGTCTT
GAATCTTGATTTACCAGTTGAGTCAATCAACGACCATGTTCTTTGGCTTCTCTTCTTCTT
TGGCTTCTTCCATTTTTACATCAACACTTCCACTAGCTTCAGCAATGTTCTCCTCTCCCT
CCACCACATTCAGATCGACGACGCCACCTCTTAACACTGATAGTTTAAACCGAAGGCGGG
AAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATAT
AGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGAT
AT
   
Sequence Name 68-K119576-0022-101-F07C-3144
GenBank Accession LN512110 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K119576-0022-101-F07C-3144
ATCCACTTCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTAAGA
GGTGGCGTCGTCGATCTGAATGTGGTGGAGGGAGAGGAGAACATTGCTGAAGCTAGTGGA
AGTGTTGATGTAAAAATGGAAGAAGCCAAAGAAGAAGAGAAGCCAAAGAACATGGTCGTT
GATTGACTCAACTGGTAAATCAAGATTCAAGACCCAAGGGGTCATGAAGTAGGAACTGTT
GTATTATCTTTGGTTACATGTATGAGACTTATTCTTCCTTTTGTAAGACCTATCTTCTGC
TTATGCAAACATTGGTAACATTTTAGGCTAACTTTGATATTCTTCATGTTTTCCTATTTT
TGGAAAATCATGAAATTATAGTTTCTCAATCAAAGCTGTTTGGTTATGCACCTCAAGCTT
TTTAACAGTGTGTGAATAGGTTCGATGAAGTCCGGTTATGTTTCCGGTTAGACGTCGTCT
GTTTCAATCTAACTACTATTCTCAATCAAACTCAAAAACTGAACTAAATAATGTAAGATA
CAAACACAAGAATGTTATAATACAAATTTGGGTTGACTTTAACATAATGGTTTCGAGTG

Last Updated on 06.12.2018 16:11