Genotyping and primer details


Genotyping and primer details for 101F07 - At2g25170
Line ID 101F07
Locus At2g25170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10723631 of line 101F07 Chr2:10723631
   
Insertion allele amplicon 3144 - 20em
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 20em: 5'-AAAGATTCCTTCGTACACTCGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10724191
   
Insertion allele amplicon 8474 - FB08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FB08: 5'-AAAGATTCCTTCGTACACTCGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10724191
   
Insertion allele amplicon 3144 - FV33
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FV33: 5'-TGAGCTTTATTGAGGTTGATGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10723117
   
wt amplicon (over InsPos) FB08 - FV33
wt amplicon start Chr2:10723117
wt amplicon end Chr2:10724191
wt amplicon size 1075 bp
Locus specific primer FV33: 5'-TGAGCTTTATTGAGGTTGATGATG-3'
2nd locus specific primer FB08: 5'-AAAGATTCCTTCGTACACTCGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11