Sequences from confirmation amplicon


Confirmation sequences for line 101G09 and hit At3g13790 are shown.
Line ID 101G09
Locus At3g13790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4534181
Primers used for PCR NA89 - 3144, show primer details
   
Sequence Name 36-K103460-0022-101-G09C-NA89
GenBank Accession LN502193 [GenBank]
Sequencing primer gene specific primer, NA89: 5'-TGTTGAAAATAAGTTTGGACGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K103460-0022-101-G09C-NA89
CGATCGGGTTAAGATAAATTCGTACCGGACAATGGTTTCAAGATGGACGGTACGGCTCCG
AGATACGATTACGGAAAGTATTACGCGTCTAAAACGTTTTTTGACTCGGCTAAGAACCGG
AGAATCTTGTGGGGTTGGACTAACGAGTCATCGTCGGTTGAGGATGATGTTGAGAAAGGC
TGGTCCGGTATTCAGGTAATTAGTTACGCTTAACTGAAACTAATAATGTGAATATAATTG
TTAGAGATTGAATCAATTTTTTGTTTCTTTCTTGATTAGACGATTCCAAGGAAAATATGG
CTTGATAGATCAGGGAAACAATTAATTCAGTGGCCGGTTAGGGAAGTTGAAAGATTACGT
ACAAAACAAGTCAAAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCC
CCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCG
AAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCC
   
Sequence Name 45-K103461-0022-101-G09C-3144
GenBank Accession LN505499 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K103461-0022-101-G09C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTTTGA
CTTGTTTTGTACGTAATCTTTCAACTTCCCTAACCGGCCACTGAATTAATTGTTTCCCTG
ATCTATCAAGCCATATTTTCCTTGGAATCGTCTAATCAAGAAAGAAACAAAAAATTGATT
CAATCTCTAACAATTATATTCACATTATTAGTTTCAGTTAAGCGTAACTAATTACCTGAA
TACCGGACCAGCCTTTCTCAACATCATCCTCAACCGACGATGACTCGTTAGTCCAACCCC
ACAAGATTCTCCGGTTCTTAGCCGAGTCAAAAAACGTTTTAGACGCGTAATACTTTCCGT
AATCGTATCTCGGAGCCGTACCGTCCATCTTGAAACCATTGTCCGGTACGAATTTATCTT
TAACCCGATCGTACGTACCAATCGTGTAATAATCATGTTTCGTGTCGTCCAAACTTATTT
TCAAC

Last Updated on 06.12.2018 16:11