Sequences from confirmation amplicon


Confirmation sequences for line 102C11 and hit At2g23290 are shown.
Line ID 102C11
Locus At2g23290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9905968
Primers used for PCR M70b - 3144, show primer details
   
Sequence Name 39-K015361-022-102-C11C-3144
GenBank Accession LN503170 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K015361-022-102-C11C-3144
GGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAA
GCCATCATACTCTACCGGTAATTTTTTATCGTTAGTTAACAACAACTTAATTTTTTCGTA
ACTGTAAATTGGATTATTTACGTGCATAATTATGTTTTCAACTGAATCAAATTGAAATAA
GTTAACTGACACCAAATTAAATATGTAAGTATATGCCGTTTTGTTTTATTATTTTAAAAT
CCAGAAAACTGAAATTTAGTTTGAACCAAACAAAATAAACTCTAAACTAAAATTTATTAA
AATATATACTATTAAACAAATTATTATGTAATGTTTTTTTAATGAATGTTAAATTATAAC
TAAACATGTACTGTATTTCTAACTATAACTAAACAAAGATATTAAACTAAAATCAAATCA
TATTACATCAAATTATAAATGCAAACCTTTTTCAAAAAATAAATTCAAATTAAAAATTCT
TATATATTGTAGTTATGTAAAACTATTTAGAAAAACTACATATTTAAGTAATTTTGCGTA
ATTTC
   
Sequence Name 40-K015361-022-102-C11C-M70b
GenBank Accession LN503533 [GenBank]
Sequencing primer gene specific primer, M70b: 5'-AATCCAATTTAGAAATTACGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K015361-022-102-C11C-M70a
TTACTTAAATATGTAGTTTTTCTAAATAGTTTTACATAACTACAATATATAAGAATTTTT
AATTTGAATTTATTTTTTGAAAAAGGTTTGCATTTATAATTTGATGTAATATGATTTGAT
TTTAGTTTAATATCTTTGTTTAGTTATAGTTAGAAATACAGTACATGTTTAGTTATAATT
TAACATTCATTAAAAAAACATTACATAATAATTTGTTTAATAGTATATATTTTAATAAAT
TTTAGTTTAGAGTTTATTTTGTTTGGTTCAAACTAAATTTCAGTTTTCTGGATTTTAAAA
TAATAAAACAAAACGGCATATACTTACATATTTAATTTGGTGTCAGTTAACTTATTTCAA
TTTGATTCAGTTGAAAACATAATTATGCACGTAAATAATCCAATTTACAGTTACGAAAAA
ATTAAGTTGTTGTTAACTAACGATAAAAAATTACCGGTAGAGTATGATGGCTTAAACCGA
AGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTC
CTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAG
TGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCA
CGATGCTCCTCGTGGGTGGG
Primers used for PCR 54wr - 8474, show primer details
   
Sequence Name 18-K118567-0022-102-C11C-54wr
GenBank Accession LN494519 [GenBank]
Sequencing primer gene specific primer, 54wr: 5'-TAAATCCGCCATGTAACTCCTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118567-0022-102-C11C-54wr
ATCTCCACCAANTCCAGAAATCTCTCTTTCCTTCTCTTCTTCTTCTTCTCTCTTCACCGG
AAACAACTCCGGGTGGAGTTGTTGACTCATTGACCCAAGGCAAAGACAACCTCAAACAAG
TCATCGGATCTTTCTCCTCCTCCTCCGGCGATGATGACTCAATCACCACTCCACCAGCTC
TCGCCGTCGGTTTAAAAACATGACAAGAAGAAGAAACCGGTAAAACAGATCCACTAGATT
GCGATTGCTCGCTGACGTCAGATCCCGTTGGACTTAACGCCGTCACCACAACAACTCCTC
CTCCACCACTCGCCCTCCGTTTTAACGGTTTTTCATCAGTTAAATTACCATCATACCCTC
CATTACCACCGAAATCACAACTCTGCCCTTCCTCTCCTCCGCCGCCTCCGCCGCTACATT
TCCGCTTCAGCGTTGAGTTCCAGTGATTCTTAATCGCGTTATCAGTGCGACCATTGAGAA
GCCGTGCAATCGTCGCCCACTTGTTACCAAACCGAGCGTGCGCTAGTATAATCGTATCAT
CTTCCTCCGCCGTGAATCCACGGTGCTCTACCTCCGGAGAAAGCTGATTACACCAACGGA
GACGGCAAGATTTACCGGAACGTCCAGGGATTGATTTGCTTATCAGAGACCAGTTCCTTG
GTCCGTGCTTCTGAACCAACGATTGTAACAGATCGTCTTCTTCAGGACTCCATGGTCCTT
TGATCCGATCCATTTCTTTCCGGGTCGAACCAGACATCGCTCGGATCTTCTTTTTAATTT
AAAACTAATCAAATCGTTTAAAACGCGTTTCTATTGTTTGGGGATTTTGGAAAAGACGAA
ACGATNTTTGTTGAGAGGATAAGTATGAAGAGGAGAGAGTATTTATACGAAGTGTGATGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGNCATGAGTAT
GATGGGTCATATGGAGAAAAAGAAAGAGT
   
Sequence Name 02-K118566-0022-102-C11C-8409
GenBank Accession LN487243 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118566-0022-102-C11C-8409
CATTGAATATATCCATCACACCTCGTATAAATACTCTCTCCTCTTCATACTTATCCTCTC
AACAAAAATCGTTTCGTTCTTTTCCAAAATCCCCAAACAATAGAAACGCGTTTTAAACGA
TTTGATTAGTTTTAAATTAAAAAGAAGATCCGAGCGATGTCTGGTTCGACCCGGAAAGAA
ATGGATCGGATCAAAGGACCATGGAGTCCTGAAGAAGACGATCTGTTACAATCGTTGGTT
CAGAAGCACGGACCAAGGAACTGGTCTCTGATAAGCAAATCAATCCCTGGACGTTCCGGT
AAATCTTGCCGTCTCCGTTGGTGTAATCAGCTTTCTCCGGAGGTAGAGCACCGTGGATTC
ACGGCGGAGGAAGATGATACGATTATACTAGCGCACGCTCGGTTTGGTAACAAGTGGGCG
ACGATTGCACGGCTTCTCAATGGTCGCACTGATAACGCGATTAAGAATCACTGGAACTCA
ACGCTGAAGCGGAAATGTAGCGGCGGAGGCGGCGGAGGAGAGGAAGGGCAGAGTTGTGAT
TTCGGTGGTAATGGAGGGTATGATGGTAATTTAACTGATGAAAAACCGTTAAAACGGAGG
GCGAGTGGTGGAGGAGGAGTTGTTGTGGTGACGGCGTTAAGTCCAACGGGATCTGACGTC
AGCGAGCAATCGCAATCTAGTGGATCTGTTTTACCGGTTTCTTCTTCTTGTCATGTTTTT
AAACCGACGGCGAGAGCTGGTGGAGTGGTGATTGAGTCATCATCGCCGGAGGAGGAGGAG
AAAGATCCGATGACTTGTTTGAGGTTGTCTTTGCCTTGGGTTCATGAGTCAACAACTCCA
CCGG

Last Updated on 06.12.2018 16:11