Sequences from confirmation amplicon


Confirmation sequences for line 102E04 and hit At1g61110 are shown.
Line ID 102E04
Locus At1g61110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22516794
Primers used for PCR 64yn - 8474, show primer details
   
Sequence Name 12-K120546-0022-102-E04C-64yn
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 64yn: 5'-TTACTAGAGTTCAATTGCGAACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K120546-0022-102-E04C-64yn
TATTTTTAAATAATTTATAGAGAATTCCCACATTTCTAGTTTTTATAAAATCTTACACAA
AAACCAAAATGAGGTATGTGCGTGATGTGTAGCATAAGAAAGGCATGACATAAGGCAGTT
CGAAAAAACCCTGCCTTTTCTCCAAAATTTAATGCCCATTGGCCGCTAATTTTGGGCCCT
ACGATTAGCCACGTGTAATTTCCTCGTCGAGGGACCCAGTTCGGTACTCATATGCTCACA
CAAACCTAACACCTATTTCTTCTCGCATCTTATATGAAACACATTCACTCTCCAAAATAA
TCTATACTTTTCCTACATATTCTCTTGACGATCATGAGACTGATTTCGTGAAAATATCGT
CATTATATCAAATTAGAAGTTGATGGAAAACATGGGGGATTCGAGCATAGGGCCGGGCCA
TCCGCATCTCCCTCCCGGGTTTCGGTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATATCCGCACGGTT
   
Sequence Name 02-K120545-0022-102-E04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K120545-0022-102-E04C-8409
TCGGATGAGCATTTCATTGAACCGAAACCCGGGAGGGAGATGCGGATGGCCCGGCCCTAT
GCTCGAATCCCCCATGTTTTCCATCAACTTCTAATTTGATATAATGACGATATTTTCACG
AAATCAGTCTCATGATCGTCAAGAGAATATGTAGGAAAAGTATAGATTATTTTGGAGAGT
GAATGTGTTTCATATAAGATGCGAGAAGAAATAGGTGTTAGGTTTGTGTGAGCATATGAG
TACCGAACTGGGTCCCTCGACGAGGAAATTACACGTGGCTAATCGTAGGGCCCAAAATTA
GCGGCCAATGGGCATTAAATTTTGGAGAAAAGGCAGGGTTTTTTCGAACTGCCTTATGTC
ATGCCTTTCTTATGCTACACATCACGCACATACCTCATTTTGGTTTTTGTGTAAGATTTT
ATAAAAACTAGAAATGTGGGAATTCTCTATAAATATATTTTAAAAAATTATCGTGTTACC
AAAATGTGTTTTTCGTTCGCAATTGAATTCTAGTA

Last Updated on 06.12.2018 16:11