Sequences from confirmation amplicon


Confirmation sequences for line 102E08 and hit At1g16020 are shown.
Line ID 102E08
Locus At1g16020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5500515
Primers used for PCR 07jn - 8474, show primer details
   
Sequence Name 16-K113214-0022-102-E08C-8409
GenBank Accession LN493503 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K113214-0022-102-E08C-8409
CATTTACATTGGGTAAGCATTCTCACCGCCCCACCCTCTTGAAATATTCTCTTCAATTTT
CAAAATTCTCAGTGATGCCTGGAAAAGATATATTGGGTATATATTAGAAGACACCAGAAA
AGAGATTCAACAAAAAAGATCGATGGGAGATGTGCTAACATCTTCAATAACTTGCTGTTT
CACGGCTGAGATGCTTAAATCTCCATTGACAATGGCATTTGTAGGCATCAGAAGAAGTAA
GGTGAGACTTTTATGCTGATAGGCAAGGAGATACATTCTTTCTTGTGTCTGCTGAAGCCA
AATTGTAGGGATGGCAGAATCAGGGGAGCCGCCAGTCTCAAGACCCCAAATATCGGTTAT
TAGAAACCCATCTTTCCCTTTTGTCCACTTATCATTTTGTAGTGGCCTAATAACATGATG
CATGTTATTACCGTTTCTTGAGTGTAGGGAATCAATTGAGCCAACGGGTGCCAGATTAGA
TCTAGAAGAGATTTCAGATGAACCAGGCCCTTTACGTAGATATGACCAAGAACTCGTATC
AGAGGATAAAGCTTTTGAGGTCAACCTCATTACCGCANATGTAAA
   
Sequence Name 16-K113215-0022-102-E08C-07jn
GenBank Accession LN493504 [GenBank]
Sequencing primer gene specific primer, 07jn: 5'-GTTTACATTTGCGGTAATGAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K113215-0022-102-E08C-07jn
GTTCTTGGTCTATCTACGTAAAGGGCCTGGTTCATCTGAAATCTCTTCTAGATCTAATCT
GGCACCCGTTGGCTCAATTGATTCCCTACACTCAAGAAACGGTAATAACATGCATCATGT
TATTAGGCCACTACAAAATGATAAGTGGACAAAAGGGAAAGATGGGTTTCTAATAACCGA
TATTTGGGGTCTTGAGACTGGCGGCTCCCCTGATTCTGCCATCCCTACAATTTGGCTTCA
GCAGACACAAGAAAGAATGTATCTCCTTGCCTATCAGCATAAAAGTCTCACCTTACTTCT
TCTGATGCCTACAAATGCCATTGTCAATGGAGATTTAAGCATCTCAGCCGTGAAACAGCA
AGTTATTGAAGATGTTAGCACATCTCCCATCGATCTTTTTTGTTGAATCTCTTTTCTGGT
GTCTTCTAATATATACCCAATATATCTTTTCCAGGCATCACTGAGAATTTTGAAAATTGA
AGAGAATATTTCAAGAGGGTGGGGCGGTGAGAATGCTTACCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT

Last Updated on 06.12.2018 16:11