Sequences from confirmation amplicon


Confirmation sequences for line 103E12 and hit At2g17000 are shown.
Line ID 103E12
Locus At2g17000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7389811
Primers used for PCR 02ht - 8474, show primer details
   
Sequence Name 31-K112586-0022-103-E12C-02ht
GenBank Accession LN500230 [GenBank]
Sequencing primer gene specific primer, 02ht: 5'-AGAGAAGAGCACTTGCCTTAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112586-0022-103-E12C-02ht
ACTCATCACATGATTAGTTTTCTCACTGCCATTGTCATCATAGTCATATGGCTGATCCTT
CTTGAAATCGCTACTTCCAAGTATCTTTTATTTCTAACTTCACAAGTTGTACTCTTAGCC
TTCATGTTTGGGAACTCTCTCAAGACCGTCTTCGAGTCAATCATCTTCCTCTTCATCATT
CACCCTTACGATGTTGGTGATCGGTTACTCATCGACACTGTAGAGGTAACTAAAAAACCA
AAATGTGTTTGTTTTTAGTCTATTTACTAAACCTTATACATAGGCTAATTTAACAAGATC
AAAATTGTAGATGGTGGTGGAGGAGGCCATTTCCGGCTTCATGTCCGGGAAATCTGTTGC
TGACAACGGTTTCATGTAGATCAAAACTGTTGTGATCAAAATACCTCATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 05-K112585-0022-103-E12C-8409
GenBank Accession LN488426 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K112585-0022-103-E12C-8409
GCATTTCATTGAATAATGAGGTATTTTGATCACAACAGTTTTGATCTACATGAAACCGTT
GTCAGCAACAGATTTCCCGGACATGAAGCCGGAAATGGCCTCCTCCACCACCATCTACAA
TTTTGATCTTGTTAAATTAGCCTATGTATAAGGTTTAGTAAATAGACTAAAAACAAACAC
ATTTTGGTTTTTTAGTTACCTCTACAGTGTCGATGAGTAACCGATCACCAACATCGTAAG
GGTGAATGATGAAGAGGAAGATGATTGACTCGAAGACGGTCTTGAGAGAGTTCCCAAACA
TGAAGGCTAAGAGTACAACTTGTGAAGTTAGAAATAAAAGATACTTGGAAGTAGCGATTT
CAAGAAGGATCAGCCATATGACTATGATGACAATGGCAGTGAGAAAACTAATCATGTGAT
GGAGTTTGTTCACTGCTGTTTTGGTGTCATTGAGTGTTAAGGCAAG

Last Updated on 06.12.2018 16:11