Sequences from confirmation amplicon


Confirmation sequences for line 103F10 and hit At3g21370 are shown.
Line ID 103F10
Locus At3g21370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7525589
Primers used for PCR VL06 - 3144, show primer details
   
Sequence Name 01-K018333-022-103-F10C-VL06
GenBank Accession LN486378 [GenBank]
Sequencing primer gene specific primer, VL06: 5'-TCCAAATGCCTAAAGAAAAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K018333-022-103-F10C-VL06
ATGTATATATATAATCAAGAATAACATTAAAATATATCAAGAGTTGATGATTTGTGTTTT
TAACCAGCCAATGACGAAATCAAGCACACGGTTTACAGTCGCTTGACCTCCTTCCACATC
TTCGGGTTCAAACCAAGCTGGACTATGAGCGATTCCGATTTTACCACCTTTGCACTTTTT
CAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGT
CCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGA
TTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGT
GGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGT
   
Sequence Name 02-K018333-022-103-F10C-3144
GenBank Accession LN486831 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K018333-022-103-F10C-3144
GGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAA
ACTATCAGTGTTTGAAAAAGTGCAAAGGTGGTAAAATCGGAATCGCTCATAGTCCAGCTT
GGTTTGAACCCGAAGATGTGGAAGGAGGTCAAGCGACTGTAAACCGTGTGCTTGATTTCG
TCATTGGCTGGTTAAAAACACAAATCATCAACTCTTGATATATTTTAATGTTATTCTTGA
TTATATATATACATAATTGCAACATTGTGTTTTTCTTTTTCTTTAGGCATTTGG

Last Updated on 06.12.2018 16:11