Sequences from confirmation amplicon


Confirmation sequences for line 103G09 and hit At5g28350 are shown.
Line ID 103G09
Locus At5g28350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:10323446
Primers used for PCR 48vf - 8474, show primer details
   
Sequence Name 14-K117806-0022-103-G09C-48vf
GenBank Accession LN492698 [GenBank]
Sequencing primer gene specific primer, 48vf: 5'-AAGAGAAAGTGCCTTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117806-0022-103-G09C-48vf
TTTTAATGAATGTGATAAACGTTTCTGAGCATTTTGTCTTTGAATGTTGTTTAATTTCAC
GTGTATGTTCGCTAATTATTGCAGATATGAGCTGCTTTTCTATCCAAGATATCACCTTGA
TCAGAGCTCTTTACTTTGTCGTAAAGTGCTGCTTGGCAAACCAATGGTGATGGATGTTTA
CCAGGATTATATACTTGTATCTTATCTTCCGTTTTTCATCCACGTCTACCATGTGAAGAT
ATATGGTGAATTGACACCTTCAAGCAAAGCAGATCTGCAGGTAGTTAGTTATTTCGGTTC
ATTTCTTCTCTGCATCATTTTCAGTAAATTATACTTTTTAACTTTCAAATGTGAGCGCTT
GAGTTGCTTTTCATGCACCTTAAAGTGATTAAATGACAAACAGCTTTCCACAGTAAGAGA
GTTGTCCATCATGACTGCAAAGAGCCATCCAGCAGCAATGGGATTTGTTCCTGATCAGCA
CCTGAGAGAAGGCGAATTGGATAATGATAATTTGTCTTCTGATAATGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 14-K117784-0022-103-G09C-8409
GenBank Accession LN492697 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117784-0022-103-G09C-8409
GCCATTTACAATTGAATATATCATTATCAGAAGACAAATTATCATTATCCAATTCGCCTT
CTCTCAGGTGCTGATCAGGAACAAATCCCATTGCTGCTGGATGGCTCTTTGCAGTCATGA
TGGACAACTCTCTTACTGTGGAAAGCTGTTTGTCATTTAATCACTTTAAGGTGCATGAAA
AGCAACTCAAGCGCTCACATTTGAAAGTTAAAAAGTATAATTTACTGAAAATGATGCAGA
GAAGAAATGAACCGAAATAACTAACTACCTGCAGATCTGCTTTGCTTGAAGGTGTCAATT
CACCATATATCTTCACATGGTAGACGTGGATGAAAAACGGAAGATAAGATACAAGTATAT
AATCCTGGTAAACATCCATCACCATTGGTTTGCCAAGCAGCACTTTACGACAAAGTAAAG
AGCTCTGATCAAGGTGATATCTTGGATAGAAAAGCAGCTCATATCTGCAATAATTAGCGA
ACATACACGTGAAATTAAACAACATTCAAAGACAAAATGCTCAGAAACGTTTATCACATT
CATGTAAAAAAGTCTGGAAGAGTTGCAAACACATTTCTACCAAAAGGATTTTTCTCTT
Primers used for PCR 32vq - 8474, show primer details
   
Sequence Name 22-K117967-0022-103-G09C-32vq
GenBank Accession LN496297 [GenBank]
Sequencing primer gene specific primer, 32vq: 5'-ATGTCAATGTCGTCAAACAATGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K117967-0022-103-G09C-32vq
AAGACAGCTACCAATGGCAGTGAAAATGGACTCTACTAGCATGCATACCTGCAGGAAATC
CTCTTGCATAAAAGGATCATCCCCTAGAGAAGGATACCAAACCTGCAATATAGAATCTCA
GCGTTATAACAAATAAAAAAATAAAAAAGACAGGGATCAAATAGAGAGTTACTGTCAGAC
ACTTAGGGGCAGGGCAACCCTATAACGAGAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAGGTGGCCTTAACAGCCCCTTTTAAACCAAAGAAATTCCCGGCTTCCCCGGGGG
CCCAAAATCAACCAAAAAAATTTCCCCAAAAAAATTTGTTTTCCCCCCCAATGGCCCACT
TGTCCCCCAAAAAATTTCACCGGATTCGGAAATTCCCCCCTCTGGCCCTTAACCAAAATA
AAAAAGGGAAGCCCCCCCTTTTCCCCTCATTAAAAAACTTCTCCGGAACCCCCAAAGGGC
ATTTAAAAGGACCCCAAAACCCAAAGGGAGTTTCCCCCCTTTGGAAAATAACAATGGAAA
AGGGTTCCTGGCCCGGAAAATCTCCGGGGATCACCATGGATTATAAGGGTCAATTGGGAA
AAAAAAAAAAATTATTTCCCATTTTTTTTTCATTTCAAAA
   
Sequence Name 06-K117966-0022-103-G09C-8409
GenBank Accession LN488998 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117966-0022-103-G09C-8409
TTGTAATCATCAATAGAGTAGAACATCCTTTCGGTTTATGCTGTCATTCTAATTGCCTTT
TGTGGTCTCCGTAGATGTCTTATACTGAGAGGAAATGGGGAGCTTTCACTTCTTGATTTG
GTTGATGGACGAGAGAGGGAACTTACAGACTCAGTTGAACTATTTTGGGTGACATGTGGT
CAATCGGAGGAGAAGACAAATCTTGTCGAGGAAGTTTCTTGGCTAGATTATGGACACCGA
GGAATGCAGGTAATTCCTCTGCTTCAAAGCTGCTTGTTATGCTCACTTTTCTTCTTCTTC
TTTTTTTTTTTTTTTTTTTTTTTTCCCCGTAAAGGGGTGCCCCGCCCCCAAATTTTCGAA
AAAAACCCCCTTTTTGAACCCCGGCTTTTTTTTTTTTTTTTTTGGTAAAACCCCAAAATT
TCTATTTGGGGGGTTGGGTCCCCTTCCCGGGGGAAAAACCTTTTTTGGAAAAAGATTTCC
CGGCGGGTTTCCCGGCAAAAAAACCCCTTTTCCCCGCCCTTGGGAACCTGGCCTTTTTGT
CCAAATTTTAATGGCCGGAAACCCCTTT

Last Updated on 06.12.2018 16:11