Sequences from confirmation amplicon


Confirmation sequences for line 104B04 and hit At5g19410 are shown.
Line ID 104B04
Locus At5g19410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6547409
Primers used for PCR KA29 - 8474, show primer details
   
Sequence Name 23-K105138-0022-104-B04C-KA29
GenBank Accession LN496550 [GenBank]
Sequencing primer gene specific primer, KA29: 5'-GAGTTTCGACTATCCAAACCAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K105138-0022-104-B04C-KA29
GATCACGAATCTCTCAACGGCTATAGAGACTCTCTTCCTCTCTCCACCCGAAACGCCACG
ATCCTCTTCGTCTCCTTCTCCCACGAAGCTGTCTTGGACGAGAACAAGACCGAGATCACT
CAACAAGCTCTCCACTCTCTCTTCTCTTTCCTTAGCCGTTGAATCTCTCAAACTGAATTT
GGCGCTATACATTAACGTCTCTTTCACGGTAAGTAGAGGCAGTAGGTCATCGTCCTGCGG
GACAAACCCGCATAGCCTTCGTAGTTGGTTATAGTCAGTGATTTTACGATTGTTCATCAA
AACCGCGGAAGACGGATCTAGTGCCTTGTGGTTCACTCTTCCTGAAATGATCTTTAAAAG
AGTGGATTTTCCTGTTCCACTCGGTCCAACCACGGCTAGGATTTTAGAGGATTCAGCAGC
TAATGAAACGGAATTGAGTATTGGGGTGTGATTGATGGTGTAGGATAGGTTAGTGACGGT
TAAAGAGTAGCGGTTAGGGGTTGGGAGTGGTGAAGAAGAAAAGGAAGAGGAAGCAGAGGA
GAATTCATCAGGAGAGTTTGATGCTGAGAAAAGAATGATGGAGTCTTCCTCGCGGTGGCT
TGTAGCCATAGCAGAAGGGTGGAAGCAGGAAGCCATGGTATGAGTATTGTTGTTCTTCTT
CATATCAAAAAGGTCTACTAATGCTTTTTATGTATGAGTAAAACTTTTTTTTCAGAATTG
GTCGACCAACAAAAGAAACAAGTGTTTAATGTGGTAGGCAGAGGTTGGTGCAACTCTTCA
AAACCTGTGTATCTAATTCTTTTTGGTTGGTCATTAATTGTTTGATTTTGCTGAGTTTAT
CGTATAAGATAAATTTATCTTACATATATGTAAACGGTATTAGTGGTTGACCAGGAATAA
AGAGTATTGGTGTATCAAACAAAAAGGTAACGTGTTTAAATCATTACTAATTGTAAATGG
CTTCATGTCCGGGGAATCTACATGGATCAGCAATGAGTATG
   
Sequence Name 07-K105139-0022-104-B04C-8409
GenBank Accession LN489264 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K105139-0022-104-B04C-8409
GCATTTCATTAGTAATGAATTTACACGTTACCTTTTTGTTTGATACACCAATACTCTTTA
TTCCTGGTCAACCACTAATACCGTTTACATATATGTAAGATAAATTTATCTTATACGATA
AACTCAGCAAAATCAAACAATTAATGACCAACCAAAAAGAATTAGATACACAGGTTTTGA
AGAGTTGCACCAACCTCTGCCTACCACATTAAACACTTGTTTCTTTTGTTGGTCGACCAA
TTCTGAAAAAAAAGTTTTACTCATACATAAAAAGCATTAGTAGACCTTTTTGATATGAAG
AAGAACAACAATACTCATACCATGGCTTCCTGCTTCCACCCTTCTGCTATGGCTACAAGC
CACCGCGAGGAAGACTCCATCATTCTTTTCTCAGCATCAAACTCTCCTGATGAATTCTCC
TCTGCTTCCTCTTCCTTTTCTTCTTCACCACTCCCAACCCCTAACCGCTACTCTTTAACC
GTCACTAACCTATCCTACACCATCAATCACACCCCAATACTCAATTCCGTTTCATTAGCT
GCTGAATCCTCTAAAATCCTAGCCGTGGTTGGACCGAGTGGAACAGGAAAATCCACTCTT
TTAAAGATCATTTCAGGAAGAGTGAACCACAAGGCACTAGATCCGTCTTCCGCGGTTTTG
ATGAACAATCGTAAAATCACTGACTATAACCAACTACGAAGGCTATGCGGGTTTGTCCCG
CAGGACGATGACCTACTGCCTCTACTTACCGTGAAAGAGACGTTAATGTATAGCGCCAAA
TTCAGTTTGAGAGATTCAACGGCTAAGGAAAGAGAAGAGAGAGTGGAGAGCTTGTTGAGT
GATCTCGGTCTTGTTCTCGTCCAAGACAGCTTCGTGGGAGAAGGAGACGAAGAGGATCGT
GGCGTTTCGGGTGGAGAGAGGAAGAGAGTCTCTATAGCCGTTGAGATGATTCGTGATCCA
CNATTCTGCTTCTGA

Last Updated on 06.12.2018 16:11