Sequences from confirmation amplicon


Confirmation sequences for line 104D01 and hit At3g12440 are shown.
Line ID 104D01
Locus At3g12440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3952371
Primers used for PCR 04yv - 8474, show primer details
   
Sequence Name 17-K122558-0022-104-D01C-04yv
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 04yv: 5'-ATTATTTGAGTTTGGGGTAGCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K122558-0022-104-D01C-04yv
CCCTTGTCTACATGCATCTCGACCGTCCAAATTTAATCGCATTTATTTCCTTAAAGATCA
GCGGTGATAAAGGGAACCAGTACTACAAATTTGTTTTAAATTTGGGATTTATTTAAGTAC
CATTTTAATTGAAATTTACTTTTCTCAGTCTTTTGCTACTATATACGCTCACAGCAAGAG
AGACCCGAGGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCG
   
Sequence Name 01-K122557-0022-104-D01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K122557-0022-104-D01C-8409
CATTTCCTCGGGTCTCTCTTGCTGTGAGCGTATATAGTAGCAAAAGACTGAGAAAAGTAA
ATTTCAATTAAAATGGTACTTAAATAAATCCCAAATTTAAAACAAATTTGTAGTACTGGT
TCCCTTTATCACCGCTGATCTTTAAGGAAATAAATGCGATTAAATTTGGACGGTCGAGAT
GCATGTAGACAAGGGTTAGTCTTCTGCATCCCTGTGACGTCGTGCGTAGGGTTTGTCCAT
TCAGCTACCCCAAACTCAAATAA

Last Updated on 06.12.2018 16:11