SimpleSearch - Line and FST details
Line specific information
| Line ID | 104D01 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N409925) |
| Segregation Analysis | 50:45:44 |
| Confirmed for Hit | At3g12440 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 709, 4812, 4849, 4868, 93056 |
| Note |
Gene hit At2g17260
| Sequence (A. th genome BLAST matches underlined) | >04-K018336-022-104-D01-8409 CCAGCATATGATTTTACAACGCTGGCTCTTGCTGCCTTGCATGCTGGAGCTTACAGCTCT GTGGGATCCTCACCTATACTGAGATGAGATACATTANGATAGATATCACGGTATATGCAT TCCGGCAACAAAGGATACCCCGCTTCTGATGTATACATGGAAATATTACTTTGCTAATTC ATGGCAATGTTATTTTTCGGTATGGTTACGCTCAAAAAAAGTCAATATAAATCGCATATC TAAAGATAATTTAGAGAATCTGGGCTATCTGATCCGGTTGCGAGTAAACCCTTTAAAGGT ACCTAGTCAAATGCTTTGGGATCCTCACTATAGCGAGAGATGGTAAGGAAACCACTACGT TTATCATCATTCATCACAATCCGCAATTTCCATCCACCAAGGAATCTATAGGACCCTCCC TATCATTGAGATAATATTAGTGCTCTAGGATCCTCCCTATAGTGAGATTGACAAAAATAT TCTTCTTGCCATAGATTCTTAATAGACTCTGAATGAACTGTTCGCCAATGTTTACGTTAA ATAAAAATATTTGGATCTATTCCCATATTTGACTCCCTGAGAATTGGGGGATCCTCTA |
| GenBank Accession | BX001143 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:7505744 - go to primer design |
| BLAST e Value | 2e-15 |
| Hit Clone Code (BAC ID) | F5J6 |
| Hit Gene Code | At2g17260 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | glutamate receptor 2 |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At5g67500
| Sequence (A. th genome BLAST matches underlined) | >04-K012183-022-104-D01-35St TTGCGTAAGGGATGACGCACAATCCCCTATCCTTCGCAGACCCTTCCTCTATATAAGGAA GTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCG GTTTAAACTATCATTGCTCCACACCGAATGCATCTAATTGATCGGCTGCTTGTCCTCGAA GTACATATGCATAGGAGATTCTCCCGATGTTGATTATCCTAACTTCGAATGATACCATTC CTTCTACTATTGGTGTAGAGATGATGTATGAGCTTTACAATTCACAACTTGCAATTACCA TTCTATATGTCCGACACCAAAAAAAATCAACGTATACAACGATTGATTGATATGAATGAT GAATTATCATTAGAGATTTAATTCTCACTCCAATTTCAATAGCTATGGGATCCTGCCTAT AATGAATCCTTTTACTACAGATGCCTTGATTTCGCCATTCCCAGATACCCATTTCATCTT CAGATTGGTCTGAGATTATGCGAAAATATACACTCATATACATAAATACTGACAGTTTGA GCTACCAATTTAGTGTAGCCCATTACCTTACATAATTCACTCAAATGCTATGGGATCCCT CCCTATAGTGAGTCGCATTAC |
| GenBank Accession | FR803063 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:26937590 - go to primer design |
| BLAST e Value | 5e-59 |
| Hit Clone Code (BAC ID) | K9I9 |
| Hit Gene Code | At5g67500 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | voltage dependent anion channel 2 |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
Gene hit At3g12440
| Sequence (A. th genome BLAST matches underlined) | >04-K018339-022-104-D01-8409 ATATGATATAATTAAACTAACAACACTCCAGTATATGATTTTACAACGCTTGTTCTTGTT GTCTTGCAGCTGGAGCTAACAGCCCTATGGGATCTCACCTATCAGTGAGATGAGACTTTA TCACCGCTGATCTTTAATGAAATAAATGCGATTAAATTTGGACGGACGAGATGCATGTAC ACAAGGGTTAGTCTTCTGCATCCCTGTGACCTCGTGCGTAGGGTTTGTCCGTTCAGCTAC CCCAAACTCAAATAATTACCGGTTTGAATTCAAAATGTTTTTTTTGTCACTTTTCGAAAG TGGAATTAAGGAGGATAATTTCAGTAAATTTTGTTCAGATTTAGCTAATGTTAAAGCTGA TTCAAATCAAAATAATCCTAAATCAAGCTTATACTTGATCTGGTTCGTGGTATTCACAAA TCGGAAAATATTCATATTATTGCATATGTTTTCGTTCATAATATATATCCAATCCACCGT AGTAGTAGTAGTATATACTTATATTATCGACTCCAAAAGATTGGTGCTTTTGTTGATTAT TCAGTTTTTGTAATAACTTGCATTGTTCATAAATTATTTCCAGAAAACAAAACAACTTGA TCTTCGACTTCGTCTATGTTTTCATGAAGTCCCCGAAAAAGACGATTCTNATGTAACATA GCTTAAGCATTGACGATTACCTATTTGATATGCTTTCTTGGGACGTCGTACTTTTTTGTC CCATATCAAATTGAAGAAATTTAAAATTTAATTCCTCATTTACCACTTTGGAATAAAACA ATAGTCCCAAAATGCCCTAACAAACTCCGAAATGGTTTTTTAAT |
| GenBank Accession | BX001144 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:3952393 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | T2E22 |
| Hit Gene Code | At3g12440 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Polynucleotidyl transferase, ribonuclease H-like superfamily protein |
| Insertion Classification | 5' region |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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