Sequences from confirmation amplicon


Confirmation sequences for line 104E01 and hit At5g63650 are shown.
Line ID 104E01
Locus At5g63650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25481923
Primers used for PCR MF04 - 8474, show primer details
   
Sequence Name 64-K031691-022-104-E01C-8409
GenBank Accession LN511122 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K031691-022-104-E01C-8409
CTTTACAATTGAATATATCCTGCCAAGGAGCTTACAGAGCCTGCTCAAGCGGCGTACTAC
AAGAGAGAAACCCCAAGCTTTTCCCTCCAAAGCGTAGAGGACATAATGAAGATCGTTGGA
GAAGCCAGGAATCCAGCTCCGTCTTCTAATGCCGTCAAGGGCTTTGATGATGATGAGGAA
GATGTGGAGGACGAGGTTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGGAGGAAGAAGAG
GAAGAGGAAGAAGATGAATACGAGAAGCATGTTAAAGAGGCCCATTCTTGTCAAGAGCCT
CCCAAAGCTTAAGAAACTCTC
   
Sequence Name 16-K031691-022-104-E01C-MF04
GenBank Accession LN493321 [GenBank]
Sequencing primer gene specific primer, MF04: 5'-TGAGAGTTTCTTAAGCTTTGGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K031691-022-104-E01C-MF04
TTCCGTATTCTCTTCTTCCTCTTCCTCTTCTTCCTCCTCTTCTTCTTCTTCTTCTTCTTC
TTCTTCAACCTCGTCCTCCACATCTTCCTCATCATCATCAAAGCCCTTGACGGCATTAGA
AGACGGAGCTGGATTCCTGGCTTCTCCAACGATCTTCATTATGTCCTCTACGCTTTGGAG
GGAAAAGCTTGGGGTTTCTCTCTTGTAGTACGCCGCTTGAGCAGGCTCTGTAAGCTCCTT
TGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11