Sequences from confirmation amplicon


Confirmation sequences for line 104E05 and hit At1g09410 are shown.
Line ID 104E05
Locus At1g09410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3036639
Primers used for PCR IS44 - 3144, show primer details
   
Sequence Name 07-K022051-022-104-E05C-3144
GenBank Accession LN489070 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K022051-022-104-E05C-3144
GTAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTT
CATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTT
TAAACTATCAGTGTTTATGATAATGTCCTTTGAAGGAAACCTGTCAAATATTAGTTTAGA
CTTCACAAGCTCGCCGCATTTAATGTACATTGTCATTAAAACAGACGCAACATACACATC
AACATCAAATTGACATCTAACCAACTGAGCATGGACCTGTTTACCATGGTGGAGGCTTGC
TAAGCTGGCGCAGACCGAAAGAATGCTTATCAATGTTGGAAAAGTGGGTCTAACTCCCTG
TTTCTGCATCAGAATAAATAAATCAAGAGCCTCCAATTCAAAACCGTTTCTTTCGTGAAT
TTTGATCACCGTTTGCCAAGATGCATCATTTCTCTCCTTCATTGAATCAAAAACCCTTCT
TGCTTTTGCTATCTCTCCTTTCTGTCCAAGCCCGGAAATCATAGCATTACACGCAATAAC
TGGCTT
   
Sequence Name 08-K022051-022-104-E05C-IS44
GenBank Accession LN489537 [GenBank]
Sequencing primer gene specific primer, IS44: 5'-AGTTATGCCAGTGAAGCCAGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K022051-022-104-E05C-IS44
TGTAATGCTATGATTTCCGGGCTTGGACAGAAAGGAGAGATAGCAAAAGCAAGAAGGGTT
TTTGATTCAATGAAGGAGAGAAATGATGCATCTTGGCAAACGGTGATCAAAATTCACGAA
AGAAACGGTTTTGAATTGGAGGCTCTTGATTTATTTATTCTGATGCAGAAACAGGGAGTT
AGACCCACTTTTCCAACATTGATAAGCATTCTTTCGGTCTGCGCCAGCTTAGCAAGCCTC
CACCATGGTAAACAGGTCCATGCTCAGTTGGTTAGATGTCAATTTGATGTTGATGTGTAT
GTTGCGTCTGTTTTAATGACAATGTACATTAAATGCGGCGAGCTTGTGAAGTCTAAACTA
ATATTTGACAGGTTTCCTTCAAAGGACATTATCATAAACACTGATAGTTTAAACCGAAGG
CGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTT
ATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
AGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGA
TGCTCCTCGTGGGTGG
Primers used for PCR FI53 - 3144, show primer details
   
Sequence Name 10-K035347-022-104-E05C-3144
GenBank Accession LN490559 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K035347-022-104-E05C-3144
CAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGTGGAATTCAATCATATCTGGTTATGCCTCACACGGTCTTGGAG
AGGAAGCATTAAAGGTTTTCTGTGAGATGCCTTTGTCTGGTAGTACTAAGCCTAACGAAG
TCACCTTCGTTGCAACTCTCTCAGCATGTAGTTACGCTGGGATGGTTGAGGAAGGGCTTA
AGATCTATGAATCTATGGAATCAGTTTTTGGGGTTAAGCCAATCACCGCGCATTATGCTT
GCATGGTAGATATGCTTGGGCGTGCAGGACGGTTTAATGAGGCAATGGAAATGATTGATA
GTATGACTGTCGAACCAGATGCTGCAGTTTGGGGTTCTCTATTAGGAGCGTGCAGAACTC
ACTCGCAGCTGGATGTGGCAGAATTTTGTGCGAAGAAACTCATAGAGATTGACCCCGGAG
A
   
Sequence Name 58-K035347-022-104-E05C-FI53
GenBank Accession LN509631 [GenBank]
Sequencing primer gene specific primer, FI53: 5'-TTCTCCGGTTCAATCTCTATGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K035347-022-104-E05C-FI53
GTTCTGCACGCTCCTAATAGAGAACCCCAAACTGCAGCATCTGGTTCGACAGTCATACTA
TCAATCATTTCCATTGCCTCATTAAACCGTCCTGCACGCCCAAGCATATCTACCATGCAA
GCATAATGCGCGGTGATTGGCTTAACCCCAAAAACTGATTCCATAGATTCATAGATCTTA
AGCCCTTCCTCAACCATCCCAGCGTAACTACATGCTGAGAGAGTTGCAACGAAGGTGACT
TCGTTAGGCTTAGTACTACCAGACAAAGGCATCTCACAGAAAACCTTTAATGCTTCCTCT
CCAAGACCGTGTGAGGCATAACCAGATATGATTGAATTCCACGGGAAACGACAATCTGAT
CCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTG
CGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCAC
TTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGT
CCAATCTTTTGG

Last Updated on 06.12.2018 16:11