Sequences from confirmation amplicon


Confirmation sequences for line 104H06 and hit At5g41550 are shown.
Line ID 104H06
Locus At5g41550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16617786
Primers used for PCR 07uh - 3144, show primer details
   
Sequence Name 44-K117093-0022-104-H06C-07uh
GenBank Accession LN505314 [GenBank]
Sequencing primer gene specific primer, 07uh: 5'-CGAGAATTCGCTCTTGTTTTATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117093-0022-104-H06C-07uh
CAACTCCACAACTTGTATGTTTACTGGTTTTGGTGCTTTCAACTTGAGAGAACTTTAAAC
CTTCATCTTCATAGTAGTCCGCTTCGCTGCTGCTACTCTCAGTTTCAAAGATGTCCAATT
CGCTGCTGCTACTCTCAGTTTCAAAGTTTTCCAATTCGCTGCTGCTACTCTTAGTTTCAA
AGTTGTCCACGTCGCTGCTGCTGCTACCCTTAGCTTCCTCCGTCATGATCTGTACACCAC
ACTCAATAATCATGTTATCGAGGTCAGGGTCATCGCTGAACTCATTTATAACACAGTTGA
ACTTAAATATAATCTCGCTCAAAATCATGTTTCGTTGATAAACCAAGCGACCGTGAAATA
TAAACAGATGTTCCGTCCCAGGAGACCAATCTGAAAGCCGTGCTAAGTCGCAGATGCGCA
TTGCAAACCCCGCTTTGCTACTTACGATGCAACTTATACTATGACATCGGTAGCCCGTAA
CTGGGATAGTACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCA
AATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGT
CATCCCTTACGTCAGTGGAGATATCAATCAATTCCAC
   
Sequence Name 28-K117092-0022-104-H06C-3144
GenBank Accession LN499014 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117092-0022-104-H06C-3144
GCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGA
TCAGATTGTCGTTTCCCGCCTTCGGTACTATCCCAGTTACGGGCTACCGATGTCATAGTA
TAAGTTGCATCGTAAGTAGCAAAGCGGGGTTTGCAATGCGCATCTGCGACTTAGCACGGC
TTTCAGATTGGTCTCCTGGGACGGAACATCTGTTTATATTTCACGGTCGCTTGGTTTATC
AACGAAACATGATTTTGAGCGAGATTATATTTAAGTTCAACTGTGTTATAAATGAGTTCA
GCGATGACCCTGACCTCGATAACATGATTATTGAGTGTGGTGTACAGATCATGACGGAGG
AAGCTAAGGGTAGCAGCAGCAGCGACGTGGACAACTTTGAAACTAAGAGTAGCAGCAGCG
AATTGGAAAACTTTGAAACTGAGAGTAGCAGCAGCGAATTGGACATCTTTGAAACTGAGA
GTAGCAGCAGCGAAGCGGACTACTATGAAGATGAAGGTTTAAAGTTCTCTCAAGTTGAAA
GCACCAAAACCAGTAAACATACAAGTTGTTGGAGTTGGTTTACGAAGCATGGTCTGAGAA
AGAAGAAGATGAATAAAACAAGACGAAATTTCTCG

Last Updated on 06.12.2018 16:11