Sequences from confirmation amplicon


Confirmation sequences for line 105C09 and hit At5g23880 are shown.
Line ID 105C09
Locus At5g23880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8055993
Primers used for PCR PB35 - 8474, show primer details
   
Sequence Name 93-K037023-022-105-C09C-8409
GenBank Accession LN515229 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K037023-022-105-C09C-8409
GCCATTGGTTCTCACGGGCCTGCATATAAAGATATATTGATTGATGCATTTGTTCCCCCA
TCCAGCAGCGTAGCTCCAATGTTCCCATATTATGATAACACATCTGAATGGGACGACTTT
GGGGAGATTATTAATCCAGATGACTATGTGATCAAGGATGAAGACATGGACCGAGGAGCA
ATGCATGTACGTTCCTTGAATTCTGGTCTTGGTCTTGGTCTTTCTTAGCTCTTGTTATAT
TTGCAAATTAGACCTCTTAATTTCCATGATGTTTGCATAGTGGTGGTTCCTTTTACGATT
TTTGTATCAAGAAGAACAAAGATGTAAGAAATCTGCATGATATGCTCTGAGGATTTGGGA
TACAGATAGGAAATCTTTTGCCATAAAGTTGCTTAGGCTCTGATTTTGGTATAAAAAAAA
AGCCTCCTTA
   
Sequence Name 94-K037023-022-105-C09C-PB35
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, PB35: 5'-ATACCAAATCAAGAGCCTAAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K037023-022-105-C09C-PB36
CCAACCTACAATTTTTTGAAAACACAAAAA

Last Updated on 06.12.2018 16:11