Sequences from confirmation amplicon


Confirmation sequences for line 105C09 and hit At5g24530 are shown.
Line ID 105C09
Locus At5g24530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8382352
Primers used for PCR MZ27 - 8474, show primer details
   
Sequence Name 07-K024285-022-105-C09C-MZ27
GenBank Accession LN489091 [GenBank]
Sequencing primer gene specific primer, MZ27: 5'-TTCGATCTTGATGATTGGCTATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K024285-022-105-C09C-MZ27
CCTTTATTAACTAAACAAATCTATAATATGTTCATAACTGTAGTAATGTTTAAATGATGA
TATTTCTTACAACACTTTATGTCTTATAAGAAAAAGAAAAAAATCATATTTTTAATCAAT
GTTAAAACTTTTAACTTCTAACAGTACCCGAACTTTTTAAAATAAATAAACGTCCAGAAT
ACATTTTTAAAAATAAATATTTTAAAAATATTTTACACTCTTCCTGCCTAAGATTTTTAG
ATGTACTTTTTGAAATCCTATAACTAATTCTCTGTAATTGTTTTAAAGATCTCAATTTAT
TGGGGTAGTTGAGTAGAGAGAAACTATTACGTAAAGACTAGAATTCTTTTCTTTTTAAGT
CCGAAGTTCATGGGCCGGATCACATTTATTTAAATGGTTTGAAACTAGGCAGGTTGATAC
AACATAAACCATCTATTTCTAAAGTTGAAGTCGAAACCATAGAGTATGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
   
Sequence Name 08-K024285-022-105-C09C-8409
GenBank Accession LN489555 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K024285-022-105-C09C-8409
GTAGATTCCCGGACATGAAGCCATTTACAATTGAATATATCATACTCTATGGTTTCGACT
TCAACTTTAGAAATAGATGGTTTATGTTGTATCAACCTGCCTAGTTTCAAACCATTTAAA
TAAATGTGATCCGGCCCATGAACTTCGGACTTAAAAAGAAAAGAATTCTAGTCTTTACGT
AATAGTTTCTCTCTACTCAACTACCCCAATAAATTGAGATCTTTAAAACAATTACAGAGA
ATTAGTTATAGGATTTCAAAAAGTACATCTAAAAATCTTAGGCAGGAAGAGTGTAAAATA
TTTTTAAAATATTTATTTTTAAAAATGTATTCTGGACGTTTATTTATTTTAAAAAGTTCG
GGTACTGTTAGAAGTTAAAAGTTTTAACATTGATTAAAAATATGATTTTTTTCTTTTTCT
TATAAGACATAAAGTGTTGTAAGAAATATCATCATTTAAACATTACTACAGTTATGAACA
TATTATAGATTTGTTTAGTTAATAAAGGGTTAGAGAATTTTCCGAGAATAATAGCCAATC
A

Last Updated on 06.12.2018 16:11