Sequences from confirmation amplicon


Confirmation sequences for line 105F08 and hit At1g11060 are shown.
Line ID 105F08
Locus At1g11060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3684618
Primers used for PCR 14ra - 8474, show primer details
   
Sequence Name 38-K116569-0022-105-F08C-14ra
GenBank Accession LN503120 [GenBank]
Sequencing primer gene specific primer, 14ra: 5'-ACCATCTTGACACTAATGGTCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K116569-0022-105-F08C-14ra
CTGCACCATTCAATTTGTGTTTATTTTTGTCCGTTGCAAAATTTATATGTATATAAATAA
GAAGAAATTACATATTTTTTTTTTTGGCCACCAAAAATTACCAAATTTGGACCCAAAAGG
TTTGGCCAAACCTTTTCCACCAAAAAAAACCCAAACCCTTTTTTCCCAATAATTTTAAAC
CCGGGAAAATCCAATTTTTCCCGGTAACCGGACCTTACCCCATTTCCCGAAACCATTGAA
AAAAAAAGGGCCCAACCAACCAAAATTTAAAACCTTCCCCCCTTGGGTAACCCCCAAAAA
AAAAACCCAAACCCAAAAAAGGTTTTTTTGGAATTCAAACCCCCCTTGAACCCCCCAAAA
TCCAACGGGGAAAATTTGGGGGCCCCTTTAAACTTTTAATGGCCAAAGTTAGTTAAAAAT
TTAAAACCTTCGGGTTCCCCCCTTAAAAATAATTTATCCGGGGGGACCCACCAAATTTAA
GGAAAATTGGAAAACCAAGGGCCAACCCAATTTGCCGCTTTTTTTTCGGGAAAATTCCCA
TTGAAAAGGGCCCCCGGCCCGGGAAACCCCCCGGGAACCCCCAGGGATTAGAGGGCCCAA
TGGGGAAAAAAAAAAAAGTTTTTCCCCTTTTTTTTTCCTTTCAAAAAGGGAAATTCCC
   
Sequence Name 09-K116568-0022-105-F08C-8409
GenBank Accession LN490356 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116568-0022-105-F08C-8409
ATTTACATTGATATATCCTGAAAAGAAAGCAGCAAATGCGATTGGCCGTTAGTTTTACAA
TTATCATTAAATTTTGTTGTTACCACAGTAATAACTAATACTAAGTGAGTAACCAGAATG
TTTTAAATTACTAACTAACTTTAGCAATTAAAAGTTAAAAAGAGCACACAAATTTTCACA
GTCTGATTTTTGGGGTTTCAAGTTGTGTCTGAATTACAACTAAACATTTATTTGTTATTG
ATTTTTTTTTTTGAGTATTACCAATGCTGGAAAGTTTTAAATTTATGCTAGTTTGAGCAT
TTTATATTCAACTGATTCAGGAAATTGTGTAAGGTACGGTATACGGTAAAATATAGAATT
TCCCAAATATAAAATTAATAGTAAAATAGGTATCTAATTTTTTTCGGTTGAAAAGGCTTT
GACAAATCATTATGTATCAAACTATGTAATTTCTTGTTGACAAAAAAAAAAAAAGGAAAT
TTCCTCCAATTTAAAACCCAAAAAATTTTGCCCCGGAAAAAAAAAAACCCAATTTTAAAG
GGTTGCAAAATTTTACCGGGGGGCCAAGGGCAAGGGACCTTT

Last Updated on 06.12.2018 16:11