Sequences from confirmation amplicon


Confirmation sequences for line 106A06 and hit At3g51550 are shown.
Line ID 106A06
Locus At3g51550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19119684
Primers used for PCR BD24 - 3144, show primer details
   
Sequence Name 33-K028098-022-106-A06C-BD24
GenBank Accession LN500863 [GenBank]
Sequencing primer gene specific primer, BD24: 5'-GGACTTTGACTATGGTTGGATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K028098-022-106-A06C-BD24
GTACTGCTCTTGAGATGTGTATAGGCTCATGTTGGAGGGAATGATATCTCGCCTTCCGCG
GATACGCGGTTTGTATAGGTCGTGGTATGATGATCAGCCTTATATATTTGGTGCAGGACT
TGGTATTCCAGAGACTGCTGATCCCAACATGACGATTAAGTATCCTACGGGGACTCCTAC
TTATGTTGCTCCTGTGGATGTTTATTCAACCGCGAGGTCTATACACTGATAGTCTTTCAA
ACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCT
CTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTG
TGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGT
TGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG
   
Sequence Name 33-K028098-022-106-A06C-3144
GenBank Accession LN500862 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K028098-022-106-A06C-3144
CTTCGCAGACCCTTCCTCCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAAGACTATC
AGTGTATAGACCTCGCGGTTGAATAAACATCCACAGGAGCAACATAAGTAGGAGTCCCCG
TAGGATACTTAATCGTCATGTTGGGATCAGCAGTCTCTGGAATACCAAGTCCTGCACCAA
ATATATAAGGCTGATCATCATACCACGACCTATACAAACCCGTATCCGCGGAAGGCGAGA
TATCATTCCCTCCAACATTGAGCCTATACACATTCTCAAGAGCAGTACTGTTATCAATAG
TAACAGAGCCAGATGATCCAACCATAGTCCAAAG

Last Updated on 06.12.2018 16:11