Sequences from confirmation amplicon


Confirmation sequences for line 106E07 and hit At5g39480 are shown.
Line ID 106E07
Locus At5g39480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15802920
Primers used for PCR IH53 - 8474, show primer details
   
Sequence Name 90-K100855-0022-106-E07C-8409
GenBank Accession LN515015 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K100855-0022-106-E07C-8409
GCCATTTACTTGAGATAATCTGCGGGTTTGATGGCTCTAACGGGTTTTTCCTCCATGGAA
GAGACAAAGAAGGCAGTTGCTTGTACCCTGGTTTTGTTATGGGCATTGAGAAGAGTTGCA
ATGTACTTCAGCTCGATGTTGTGCCGAGGCAAGAGAAGAGTTCGTGCAATGAGATTGAGA
GAGGAGCCTCAAGAGAGGAGGGAGAGATTCCATTTTGGATGCTCGCTTTTAGTGATAGAA
AAAATTTGCTCAATATTGTGACAAGCCATGTAAGTCTACATGTGGTGGAACCATTAAATG
AAATGTTATTTCCCACATTGAAATATGATGAAGCAATGCTGGTTGAACGCAGAACCATTC
TCCTTAAAATGCACAAGTTTGGTGGAAATTGGAAGAACATGAATTTGGAGGAGGATGATC
AGTTGTGTTACAATCCCATGCAAGT
   
Sequence Name 90-K100861-0022-106-E07C-IH53
GenBank Accession LN515016 [GenBank]
Sequencing primer gene specific primer, IH53: 5'-CGTTTATTTTTACTTGCATGGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K100861-0022-106-E07C-IH53
TCTCTCAATTATGTTCTTCAATTTCCACCAAACTTGTGCATTTTAAGGAGAATGGTTCTG
CGTTCAACCAGCATTGCTTCATCATATTTCAATGTGGGAAATAACATTTCATTTAATGGT
TCCACCACATGTAGACTTACATGGCTTGTCACAATATTGAGCAAATTTTTTCTATCACTA
AAAGCGAGCATCCAAAATGGAATCTCTCCCTCCTCTCTTGAGGCTCCTCTCTCAATCTCA
TTGCACGAACTCTTCTCTTGCCTCGGCACAACATCGAGCTGAAGTACATTGCAACTCTTC
TCAATGCCCATAACAAAACCAGGGTACAAGCAACTGCCTTCTTTGTCTCTTCCATGGAGG
AAAAACCCGTTAGAGCCATCANACCCGCAGATTATCTCANTGTANATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
Primers used for PCR 10lf - 8474, show primer details
   
Sequence Name 80-K113838-0022-106-E07C-10lf
GenBank Accession LN513974 [GenBank]
Sequencing primer gene specific primer, 10lf: 5'-CTCACTATGTTCTTCAGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K113838-0022-106-E07C-10lf
GTCTACCTTTAGGACATTTCCCAGCTGGGCAGCCAATCGTTCCTCCCCACAAACCAGCAT
ACGCTTCTTGATCGACGGCTTTCTTAACTGGTAGTTTATAAAGTAAAAAGTGGTGAAAGT
GCATGTATGGCCACCCTTTATAATAAGACATCTCACTGTAGGAAGCTGTGAGACCAAGAC
CGAATGTATCACCAGAGCTAAGAAAGCTTTGGCGATTCGAAACTCTAATTTCACTCTGCG
AAGGTGTTGTGTTCTTTGAAGAACTAGACTCCCCTAAAACATTCGTGTTCTCCGTTGGTG
TGACCTCTATCAGCTCCTCTTCATCAAAAAAATAGTCATCACCGAGATTTTCCAACATCT
CGTTTATTTTTACTTGCATGGGATTGTAACACAACTGATCATCCTCCTCCAAATTCATGT
TCTTCCAATTTCCACCAAACTTGTGCATTTTAAGGAGAATGGTTCTGCGTTCAACCAGCA
TTGCTTCATCATATTTCAATGTGGGAAATAACATTTCATTTAATGGTTCCACCACATGTA
GACTTACATGGCTTGTCACAATATTGAGCAAATTTTTTCTATCACTAAAAGCGAGCATCC
AAAATGGAATCTCTCCCTCCTCTCTTGAGGCTCCTCTCTCAATCTCATTGCACGAACTCT
TCTCTTGCCTCGGCACAACATCGAGCTGAAGTACATTGCAACTCTNCTCAATGCCCATAA
CAAAACCAGGGTACAAGCAACTGCCTTCTTTGTCTCTTCCATGGAGGAAAAACCCGTTAG
AGCCATCAAACCCGCAGATTATCTCAATGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAATGT
   
Sequence Name 89-K113839-0022-106-E07C-8409
GenBank Accession LN514972 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K113839-0022-106-E07C-8409
TTGAGATAATCTGCGGGTTTGATGGCTCTAACGGGTTTTTCCTCCATGGAAGAGACAAAG
AAGGCAGTTGCTTGTACCCTGGTTTTGTTATGGGCATTGAGAAGAGTTGCAATGTACTTC
AGCTCGATGTTGTGCCGAGGCAAGAGAAGAGTTCGTGCAATGAGATTGAGAGAGGAGCCT
CAAGAGAGGAGGGAGAGATTCCATTTTGGATGCTCGCTTTTAGTGATAGAAAAAATTTGC
TCAATATTGTGACAAGCCATGTAAGTCTACATGTGGTGGAACCATTAAATGAAATGTTAT
TTCCCACATTGAAATATGATGAAGCAATGCTGGTTGAACGCAGAACCATTCTCCTTAAAA
TGCACAAGTTTGGTGGAAATTGGAAGAACATGAATTTGGAGGAGGATGATCAGTTGTGTT
ACAATCCCATGCAAGTAAAAATAAACGAGATGTTGGAAAATCTCGGTGATGACTATTTTT
TTGATGAAGAGGAGCTGATAGAGGTCACACCAACGGAGAACACGAATGTTTTAGGGGAGT
CTAGTTCTTCAAAGAACACAACACCTTCGCAGAGTGAAATTAGAGTTTCGAATCGCCAAA
GCTTTCTTAGCTCTGGTGATACATTCGGTCTTGGTCTCACAGCTTCCTACAGTGAGATGT
CTTATTATAAAGGGTGGCCATACATGCACTTTCACCACTTTTTACTTTATAAACTACCAG
TTAAGAAAGCCGTCGATCAAGAAGCGTATGCTGGTTTGTGGGGAGGAACGATTGGCTGCC
CAGCTGGGAAATGTCCTAAAGGTAAGACGGANAAGTCTCTTTACTTACTGATGCTCACTT
ATGAAGATCTG
Primers used for PCR 11lf - 8474, show primer details
   
Sequence Name 81-K113838-0022-106-E07C-11lf
GenBank Accession LN514096 [GenBank]
Sequencing primer gene specific primer, 11lf: 5'-TCCAACATCTCGTTTATTTTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K113838-0022-106-E07C-11lf
AACTTGTGCATTTTAAGGAGAATGGTTCTGCGTTCAACCAGCATTGCTTCATCATATTTC
AATGTGGGAAATAACATTTCATTTAATGGTTCCACCACATGTAGACTTACATGGCTTGTC
ACAATATTGAGCAAATTTTTTCTATCACTAAAAGCGAGCATCCAAAATGGAATCTCTCCC
TCCTCTCTTGAGGCTCCTCTCTCAATCTCATTGCACGAACTCTTCTCTTGCCTCGGCACA
ACATCGAGCTGAAGTACATTGCAACTCTTCTCAATGCCCATAACAAAACCAGGGTACAAG
CAACTGCCTTCTTTGTCTCTTCCATGGAGGAAAAACCCGTTAGAGCCATCAAACCCGCAG
ATTATCTCAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATTC
   
Sequence Name 90-K113839-0022-106-E07C-oriL
GenBank Accession LN515056 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K113839-0022-106-E07C-oriL
AGATAATCTGCGGGTTTGATGGCTCTAACGGGTTTTTCCCTCCATGGAAGAGACAAAGAA
GGCAGTTGCTTGTACCCTGGTTTTGTTATGGGCATTGAGAAGAGTTGCAATGTACTTCAG
CTCGATGTTGTGCCGAGGCAAGAGAAGAGTTCGTGCAATGAGATTGAGAGAGGAGCCTCA
AGAGAGGAGGGAGAGATTCCATTTTGGATGCTCGCTTTTAGTGATAGAAAAAATTTGCTC
AATATTGTGACAAGCCATGTAAGTCTACATGTGGTGGAACCATTAAATGAAATGTTATTT
CCCACATTGAAATATGATGAAGCAATGCTGGTTGAACGCAGAACCATTCTCCTTAAAATG
CACAAGTTTGGTGGAAATTGGAAGAACATGAATTTGGAGGAGGATGATCAGTTGTGTTAC
AATCCCATGCAAGTAAAAATAAC
Primers used for PCR 66lf - 8474, show primer details
   
Sequence Name 80-K113840-0022-106-E07C-66lf
GenBank Accession LN513975 [GenBank]
Sequencing primer gene specific primer, 66lf: 5'-AAAGCTTTGGCGATTCGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K113840-0022-106-E07C-66lf
CTAGACTCCCCTAAAACATTCGTGTTCTCCGTTGGTGTGACCTCTATCAGCTCCTCTTCA
TCAAAAAAATAGTCATCACCGAGATTTTCCAACATCTCGTTTATTTTTACTTGCATGGGA
TTGTAACACAACTGATCATCCTCCTCCAAATTCATGTTCTTCCAATTTCCACCAAACTTG
TGCATTTTAAGGAGAATGGTTCTGCGTTCAACCAGCATTGCTTCATCATATTTCAATGTG
GGAAATAACATTTCATTTAATGGTTCCACCACATGTAGACTTACATGGCTTGTCACAATA
TTGAGCAAATTTTTTCTATCACTAAAAGCGAGCATCCAAAATGGAATCTCTCCCTCCTCT
CTTGAGGCTCCTCTCTCAATCTCATTGCACGAACTCTTCTCTTGCCTCGGCACAACATCG
AGCTGAAGTACATTGCAACTCTTCTCAATGCCCATAACAAAACCAGGGTACAAGCAACTG
CCTTCTTTGTCTCTTCCATGGAGGAAAAACCCGTTAGAGCCATCAAACCCGCAGATTATC
TCAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCA
   
Sequence Name 72-K113841-0022-106-E07C-8409
GenBank Accession LN512762 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K113841-0022-106-E07C-8409
TTACTTGAGATATCTGCGGGTTTGATGGCTCTAACGGGTTTTTCCTCCATGGAAGAGACA
AAGAAGGCAGTTGCTTGTACCCTGGTTTTGTTATGGGCATTGAGAAGAGTTGCAATGTAC
TTCAGCTCGATGTTGTGCCGAGGCAAGAGAAGAGTTCGTGCAATGAGATTGAGAGAGGAG
CCTCAAGAGAGGAGGGAGAGATTCCATTTTGGATGCTCGCTTTTAGTGATAGAAAAAATT
TGCTCAATATTGTGACAAGCCATGTAAGTCTACATGTGGTGGAACCATTAAATGAAATGT
TATTTCCCACATTGAAATATGATGAAGCAATGCTGGTTGAACGCAGAACCATTCTCCTTA
AAATGCACAAGTTTGGTGGAAATTGGAAGAACATGAATTTGGAGGAGGATGATCAGTTGT
GTTACAATCCCATGCAAGTAAAAATAAACGAGATGTTGGAAAATCTCGGTGATGACTATT
TTTTTGATGAAGAGGAGCTGATAGAGGTCACACCAACGGAGAACACGAATGTTTTAGGGG
AGTCTAGTTCTTCAAAGAACACAACACCTTCGCAGAGTGAAATTAGAGTTTCGAATC

Last Updated on 06.12.2018 16:11