Sequences from confirmation amplicon


Confirmation sequences for line 106E10 and hit At5g41070 are shown.
Line ID 106E10
Locus At5g41070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16439867
Primers used for PCR 07us - 8474, show primer details
   
Sequence Name 44-K117737-0022-106-E10C-07us
GenBank Accession LN505322 [GenBank]
Sequencing primer gene specific primer, 07us: 5'-AGCAAAATCCTGAGTTCCTAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117737-0022-106-E10C-07us
TCGGATATGACCAAACGTCATTCTCGCAAACCATAATGTTTATATGGTCCAAAATCTTTA
CACAAAGAAAAATGTAACTTGTGAAATTTATAATTTCGAATTATTAAGTTTATTTGTTGG
AACCGACGTTACAGAGCAAAACCATTAATGATGAAGAGTGGGAAGCAGAGCAGAGCATTC
GGCGCATTAATGGAGGAAGACAAAACAAAACCCATTAATTGTTTTCTTGCAAACTCCCAT
TTCTCTTCTTCATCTTCTTCCCTAATTAATGCTCTCTCTTTGTTTTCGTTCTCTGTGCAA
TTCGCAAGCATTCAAAGTTCATGTCGGACTTTTAGAAAATTCAAACACTTCAAAAAAAAC
AAGAACACATTCAAGAAACGGAAACATACTGGTCTATAATTTATCTATGTGACAAACACT
TGACAAGGATTTAATTTGAGATTAACCCATTAACCAAATTTAGCTAACTATCATGGGTTT
GATCCAAAATTCTCGATTCTGAACCAAGTTTGAGAAAGAGCAGCAATGAGTATGATGATC
AGCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 12-K117736-0022-106-E10C-8409
GenBank Accession LN491771 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117736-0022-106-E10C-8409
CTGATCATCATACTCATTGCTGCTCTTTCTCAAACTTGGTTCAGAATCGAGAATTTTGGA
TCAAACCCATGATAGTTAGCTAAATTTGGTTAATGGGTTAATCTCAAATTAAATCCTTGT
CAAGTGTTTGTCACATAGATAAATTATAGACCAGTATGTTTCCGTTTCTTGAATGTGTTC
TTGTTTTTTTTGAAGTGTTTGAATTTTCTAAAAGTCCGACATGAACTTTGAATGCTTGCG
AATTGCACAGAGAACGAAAACAAAGAGAGAGCATTAATTAGGGAAGAAGATGAAGAAGAG
AAATGGGAGTTTGCAAGAAAACAATTAATGGGTTTTGTTTTGTCTTCCTCCATTAATGCG
CCGAATGCTCTGCTCTGCTTCCCACTCTTCATCATTAATGGTTTTGCTCTGTAACGTCGG
TTCCAACAAATAAACTTAATAATTCGAAATTATAAATTTCACAAGTTACATTTTTCTTTG
TGTAAAGATTTTGGACCATATAAACATTATGGTTTGCGAGAATGACGTTTGTGTCATTAT
CCGATTTAGATCCGTATAATCTATGTTGTTAGGAACTCAGGATTTTGCT

Last Updated on 06.12.2018 16:11