Sequences from confirmation amplicon


Confirmation sequences for line 106F01 and hit At5g40390 are shown.
Line ID 106F01
Locus At5g40390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16161939
Primers used for PCR MS22 - 8474, show primer details
   
Sequence Name 05-K016383-022-106-F01C-8409
GenBank Accession LN488140 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K016383-022-106-F01C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGCGTCGAGGTTAAACCCGATGAATGAACCG
GCAGAGACGTCGAGTGGTACACCGTCTTTGTCGACTAAGTAAGGTGAAGAGGTGAGAGTA
ACGTTAACCGGAACATCGGTTAAGACGACTTGACCATTGGCTAAGAGAGTGGAATCTTCT
AACCGGAATTTTTCGGTAAAGTCAACGCCATTGATACCGGAATCGGACTTGGTCAAACAC
GGCGAAGCCATTTTGCCGATATGCAAGATTTTGGACAAAGGATTGAGAATGTTTTTTAGG
TTAAGATGGTAGAGAGTAATTTATAGAGTTTATGAAACTTAAAAACTTTCCCGAATTTGA
TTATATGATAAGATTATGGATTGAAAGAAAGATAGGAAATGTCATTAGATACTTATAGAA
TTATTGGTCGACATGTAAGGAATGAGTAAGAGAGTGAAACCTATACTTTCTTCGTTTTTG
TTAATTTAATTACTTCTTTATATATTTTGATATATTTGTGGGGCTGTTTGATTTGCTACC
TTTAGATTTGGTCGTTGGTGATTTAATACGAAGGGAAATAAAATATTGTATACATGGTTC
TA
   
Sequence Name 06-K016383-022-106-F01C-MS22
GenBank Accession LN488583 [GenBank]
Sequencing primer gene specific primer, MS22: 5'-GAGCCATGTGACTAGAACCATGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K016383-022-106-F01C-ms22
TACAATATTTTATTTCCCTTCGTATTAAATCACCAACGACCAAATCTAAAGGTAGCAAAT
CAAACAGCCCCACAAATATATCAAAATATATAAAGAAGTAATTAAATTAACAAAAACGAA
GAAAGTATAGGTTTCACTCTCTTACTCATTCCTTACATGTCGACCAATAATTCTATAAGT
ATCTAATGACATTTCCTATCTTTCTTTCAATCCATAATCTTATCATATAATCAAATTCGG
GAAAGTTTTTAAGTTTCATAAACTCTATAAATTACTCTCTACCATCTTAACCTAAAAAAC
ATTCTCAATCCTTTGTCCAAAATCTTGCATATCGGCAAAATGGCTTCGCCGGGTTTGACC
AAGTCCGATTCCGGTATCAATGGCGTTGACTTTACCGAAAAATTCCGGTTAGAAGATTCC
ACTCTCTTAGCCAATGGTCAAGTCGTCTTAACCGATGTTCCGGTTAACGTTACTCTCACC
TCTTCACCTTACTTAGTCAACAAAAACGGTGTACCACTCGACGTCTCTGCCGGTTCATTC
ATCGGGTTTAACCTCGACGCAATTGTAAATGGCTTCTTGTCCGGGAAATCTACTTGGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCATTTTTTTTTC
AATTCAAAAA

Last Updated on 06.12.2018 16:11