Sequences from confirmation amplicon


Confirmation sequences for line 106F05 and hit At1g58030 are shown.
Line ID 106F05
Locus At1g58030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21464484
Primers used for PCR 15ra - 8474, show primer details
   
Sequence Name 74-K115895-0022-106-F05C-15ra
GenBank Accession LN513133 [GenBank]
Sequencing primer gene specific primer, 15ra: 5'-TCTGAGTTCCATAGATCAGGATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K115895-0022-106-F05C-15ra
GAGTATGCTAATAAATTGGTACCTACATAAACTCTTAGCTTTTTTTCTGCATATGGAAGG
CCTTATATGTCTTGTGGTTGAAATTCTTTTTGAATAGGTTACATGTGCCCGTTTGTTCCC
CTCCTACCTATCATCTGCATTCTCATCAACATGTACCTTTTGGTTAACCTTGGGTTAGTA
TCTCTTTCACTTACTTGCGACTTTCATTTACCGTTTATTTTCTCTTGTTTCCACAGCTAT
TTTTATAACGGAATCTAACTTCAAGACGCTGTTTAACCTTAACCATTTAGGTTTAAAGAC
CGAGTTTGTAAAGCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCAGCACGGTTATT
   
Sequence Name 44-K115894-0022-106-F05C-8409
GenBank Accession LN505294 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K115894-0022-106-F05C-8409
CATTTCAGCTTTACAAACTCGGTCTTTAAACCTAAATGGTTAAGGTTAAACAGCGTCTTG
AAGTTAGATTCCGTTATAAAAATAGCTGTGGAAACAAGAGAAAATAAACGGTAAATGAAA
GTCGCAAGTAAGTGAAAGAGATACTAACCCAAGGTTAACCAAAAGGTACATGTTGATGAG
AATGCAGATGATAGGTAGGAGGGGAACAAACGGGCACATGTAACCTATTCAAAAAGAATT
TCAACCACAAGACATATAAGGCCTTCCATATGCAGAAAAAAAGCTAAGAGTTTATTGTAT
GGTACCAATTTATATAGCATTACCTCCAGAATGTCCAAAAGTGTGTCTCGCATCATCCTG
ATCTATGGAAACTCAG

Last Updated on 06.12.2018 16:11