Sequences from confirmation amplicon


Confirmation sequences for line 106H05 and hit At1g03080 are shown.
Line ID 106H05
Locus At1g03080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:733602
Primers used for PCR MZ80 - 3144, show primer details
   
Sequence Name 11-K026766-022-106-H05C-3144
GenBank Accession LN490956 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K026766-022-106-H05C-3144
CGTAGGGATGCACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAG
TTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGG
TTTAAACTATCAGTGTTTGCAATGTCTGCAGATTTTAACTTATCACCAAGCTCTCTTACC
TCTTCCTCAAGCTTACACTTGACAATACTGAGTCTGTCTAGATCCTCGTTTAGTTTCATT
GCTCCTGAAAGCTTTTCCAAGATGACATCCTCCAACAGAATGATGAGATTGCTTTGATAT
ATAGTCTCCGACAGAAGCAAAGAGATATCATCTTCTAGTTTACATTTCTCCTCCTCCAAC
TGCAATGTTGACTTTGTCAAGTATGCTTTCTCATCCAACGTTTTGTTATTATCTCCCTGT
AGAATGGTGTAGTCATCTCGTAACTGCAACACCTGCCTGTGAAAATCCTCAATTTCCACC
ATCAAAACCTTTTCTCTGTTGACTCCTTGATTAACTTTTGTGGTCAATTCTCCGTTCACG
AAGATAA
   
Sequence Name 12-K026766-022-106-H05C-MZ80
GenBank Accession LN491414 [GenBank]
Sequencing primer gene specific primer, MZ80: 5'-AGACCCAGAAACTTATCTTCGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K026766-022-106-H05C-MZ80
AATTGACCACAAAAGTTAATCAAGGAGTCAACAGAGAAAAGGTTTTGATGGTGGAAATTG
AGGATTTTCACAGGCAGGTGTTGCAGTTACGAGATGACTACACCATTCTACAGGGAGATA
ATAACAAAACGTTGGATGAGAAAGCATACTTGACAAAGTCAACATTGCAGTTGGAGGAGG
AGAAATGTAAACTAGAAGATGATATCTCTTTGCTTCTGTCGGAGACTATATATCAAAGCA
ATCTCATCATTCTGTTGGAGGATGTCATCTTGGAAAAGCTTTCAGGAGCAATGAAACTAA
ACGAGGATCTAGACAGACTCAGTATTGTCAAGTGTAAGCTTGAGGAAGAGGTAAGAGAGC
TTGGTGATAAGTTAAAATCTGCAGACATTGCAAACACTGATAGTTTAAACCGAAGGCGGG
AAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATAT
AGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGAT
ATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGC
Primers used for PCR FI56 - 8474, show primer details
   
Sequence Name 54-K028245-022-106-H05C-8409
GenBank Accession LN508363 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K028245-022-106-H05C-8409
TGATCCATGTAGATTTCCCGGACATGAAGCCATTTACACTTCAAGCTCTCTGCCAGATCA
GCCAATGTTCATTTGGAACATGAAATAGCTAATGTGAAGGTTCAGAAGGAGAAAGAGCTT
TTAGAAGCAATGCTGATGATCTCCATCATGCAAAATGAAAAATCTGAATTATCTAAGGCA
GTGGAGGGTTTAGAATGTAGATACAAGGAAGCTAAAGCGATAGAAGAGGATAGGGATAAG
CAGGTCTTGAGACTCCGTGGAGATTACGATGAGCAGGTTAAGAAGAATAGTCATTCGAAC
GAAGCTAATCTGAAATTGGAAGCTGACTTGATGAACTTACTCATGGAACTCGAAGAAATC
AAAGTGGAGAAGGAAAATCTGAACCAGGAGCTTT
   
Sequence Name 55-K028245-022-106-H05C-FI56
GenBank Accession LN508698 [GenBank]
Sequencing primer gene specific primer, FI56: 5'-TGTAAAAAGCTCCTGGTTCAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K028245-022-106-H05C-FI56
CCACTTTGATTTCTTCGAGTTCCATGAGTAAGTTCATCAAGTCAGCTTCCAATTTCAGAT
TAGCTTCGTTCGAATGACTATTCTTCTTAACCTGCTCATCGTAATCTCCACGGAGTCTCA
AGACCTGCTTATCCCTATCCTCTTCTATCGCTTTAGCTTCCTTGTATCTACATTCTAAAC
CCTCCACTGCCTTAGATAATTCAGATTTTTCATTTTGCATGATGGAGATCATCAGCATTG
CTTCTAAAAGCTCTTTCTCCTTCTGAACCTTCACATTAGCTATTTCATGTTCCAAATGAA
CATTGGCTGATCTGGCAGAGAGCTTGAAGTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11