Sequences from confirmation amplicon


Confirmation sequences for line 107A06 and hit At4g11220 are shown.
Line ID 107A06
Locus At4g11220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6839022
Primers used for PCR BH71 - 3144, show primer details
   
Sequence Name 10-K104381-0022-107-A06C-BH71
GenBank Accession LN490638 [GenBank]
Sequencing primer gene specific primer, BH71: 5'-GAATAAGAGAGAAGGCAGAAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104381-0022-107-A06C-BH71
CTATGTATGCCAATGTCAAGAAACTATAGCAGCCGCCCAAGATCGATAGAACCCACAATC
CGGCAATAGCCTAATTCAATCAAACAGTACAATAAGTGTCAAACTTAAGTGAGACATCGA
GAGAGGAGTAACGAGTAAGTCCAGGACAGTTTAAACATACAGAGAGGAACTTCTTGATAT
CCCTCCCCGACGCAATCTCACGAAGAGAAGAGATCCCACGGTTGATTTCGATTCTAAGAC
CCGAAGCAAGCTGAAGAAGAGGTTCTTCAGGGATATGAACTTCAGGAATCTTTGGTGGAG
ACCTGAAAGAGAATGAAAATTTGGTAACGAAGATGAGTCAAATAAGCTTGACAACCTTGA
CCTGTTTCATATGATTGACCACAAAACTTACTTGTGAATGAACATAGTGGCATTAGACCA
TAGAAACAACACAGCAAGAGCAACGATCATAACGTGACATAACAGAGTAAGAAGATGATA
CTCCATCAATTCAAATAGCACCCATGCCACTGTAGCACCGCCAAATACACCTCCTGACAT
TTTCTTGTCCTTCCACATAAATATATCTGCCGCTGCAATTAAAAAGATCACCAATCCACA
TCAATCCCTTACGTCAGTGGAGATTC
   
Sequence Name 26-K104382-0022-107-A06C-3144
GenBank Accession LN497908 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104382-0022-107-A06C-3144
TGATCTTTTTATTGCAGCGGCAGATATATTTATGTGGAAGGACAAGAAAATGTCAGGAGG
TGTATTTGGCGGTGCTACAGTGGCATGGGTGCTATTTGAATTGATGGAGTATCATCTTCT
TACTCTGTTATGTCACGTTATGATCGTTGCTCTTGCTGTGTTGTTTCTATGGTCTAATGC
CACTATGTTCATTCACAAGTAAGTTTTGTGGTCAATCATATGAAACAGGTCAAGGTTGTC
AAGCTTATTTGACTCATCTTCGTTACCAAATTTTCATTCTCTTTCAGGTCTCCACCAAAG
ATTCCTGAAGTTCATATCCCTGAAGAACCTCTTCTTCAGCTTGCTTCGGGTCTTAGAATC
GAAATCAACCGTGGGATCTCTTCTCTTCGTGAGATTGCGTCGGGGAGGGATATCAAGAAG
TTCCTCTCTGTATGTTTAAACTGTCCTGGACTTACTCGTTACTCCTCTCTCGATGTCTCA
CTTAAGTTTGACACTTATTGTACTGTTTGATTGAATTAGGCTATTGCCGGATTGTGGGTT
CTATCGATCTTGGGCGGCTGCTATAGTTTCTTGACATTGGCATACATAGGTAACACTTAC
TGTTGCTAAAAGTATTTTGCTTCTGCCT
Primers used for PCR BH70 - 8474, show primer details
   
Sequence Name 41-K118809-0022-107-A06C-BH70
GenBank Accession LN504265 [GenBank]
Sequencing primer gene specific primer, BH70: 5'-GTTTATCGTTTGTTCGGAAGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118809-0022-107-A06C-BH70
AACGTATGTTACTCTGATCCCGTTTCGATATTTGTTCTCAGTTTTTTAAAACGAATTCGT
TTGCGTTGGCTTGATCTGTTTGATTTTCTCACTAGTTTAAGGGATCCGTGAAGAAGATCG
TTTGAATCTACATAGAGGTTTACATTGATTTGAATTCGATTTCGATTTTGATTTCTGACT
AGTTTGAGAGAATTTGTGATGGAATTGATTTTTTTTTTTTGGGAAAGGGGTAAAAGGATC
TGGGGAAATTCTGGTATTTATGGAGGATAGGGTTCTTATTTCGGGATATTTTCAATTGAA
AAGGGCTTCTGGCCCGGGAAATCTCCTGGGATCACCAAGGAGTATGAGGGTCAATATGGA
AAAAAAAAAAGAGTATTTCCCATTTTTTTTTCATTTCAAAATGGTAAATCCCCCCA
   
Sequence Name 09-K118808-0022-107-A06C-8409
GenBank Accession LN490413 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118808-0022-107-A06C-8409
ATTGAATATATCCTGAAATAAGAACCATATCATCAATAACTACAAGAATCTCACAAGATC
ATTATACACATTTCCAAAAAAAAAAAAATCATTTCCTTCCCAATTTCTCTCAAACTAGTC
AAAATTCAAATTCGAATTCGATTTCAATTCATGGTAACCCTCTTTGTAAATTCAAACGAT
CTTCTTCCGGGACCCTTTAACCTGGGGGGAAATTCAAACAGATCAGGCCACCGCAAACAA
TTTCTTTTTAAAAACCGGAAAACAATTTTCGAACGGGGATCAAAGTAAACTTCCTTTTCC
CCCCCCCAAAAACCTTAGAACGGGTTTCTTTTTCCCAAAAAACAATAA

Last Updated on 06.12.2018 16:11