Sequences from confirmation amplicon


Confirmation sequences for line 107E05 and hit At5g50950 are shown.
Line ID 107E05
Locus At5g50950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20730718
Primers used for PCR CP05 - 8474, show primer details
   
Sequence Name 22-K031183-022-107-E05C-CP05
GenBank Accession LN495994 [GenBank]
Sequencing primer gene specific primer, CP05: 5'-TAACAAAATGACGTCAAAGGTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K031183-022-107-E05C-CP05
ATTGCACATTTCAAAGCAACTCCCAAATATAAATAGTACCACATTGAACTGAGGTCAAGA
GTGTTCCCTGACACCATTTAGGTCAGAGCAAGTATCATCTTAAAATATTGCAATTTAGTG
ACTACATCAAATGAGTCACCTTAGATTCCAAAGAGCTATGCAAATTTTTCAAACTAGGGA
TTAGCCTCGAAGTAATCTCGGTTGCAGCTGCAATGTGCATGACCTATACAAAACCAAAAT
TATACGACCGTCAAGTAGTACTTGACAGAGCAGTGAGCATACATATAGGAAATAAAAGCA
ATCGGTTACACGAACACCGTAAGCAATTGAATGAACGTTAAGCATGCAAAATTATTGGTA
ATGACTAATGAGAATGCAAACTAAAGCTTACAGTTGGAAAAGTGTCATTAGAAGATTGTG
ATCTGTTCACATGGTCATTTGGGTGCACAATTTTTTCACCACGTTTGTGACCAAGAATCT
CAGCTGCTCTATTGGCAATGACCTGTAAAAAGCACAAAATGATCTGTTTAATTACCTCGG
ACGTCTGGTGGTTAAGTATCTAAAAAATCATTTTTGACATTGTAATTAGTCTTAACTCTT
AAGCCAAAATGCCTTGATTGTGTTGTTTGATCCCTACTACCATCATATGATGCCCCACCA
ATATGCAATATTATTTCGTTATATTAACACACAATGGCGCAGACCAAACCATGTACTTTC
ATTGCCATTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTANTTATAGGCGAAAGCAATAACAAATTNATCTAATTCG
   
Sequence Name 21-K031183-022-107-E05C-8409
GenBank Accession LN495548 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K031183-022-107-E05C-8409
CATTTACAATTGAATATATCCTGTAAATGGCAATGAAAGTACATGGTTTGGTCTGCGCCA
TTGTGTGTTAATATAACGAAATAATATTGCATATTGGTGGGGCATCATATGATGGTAGTA
GGGATCAAACAACACAATCAAGGCATTTTGGCTTAAGAGTTAAGACTAATTACAATGTCA
AAAATGATTTTTTAGATACTTAACCACCAGACGTCCGAGGTAATTAAACAGATCATTTTG
TGCTTTTTACAGGTCATTGCCAATAGAGCAGCTGAGATTCTTGGTCACAAACGTGGTGAA
AAAATTGTGCACCCAAATGACCATGTGAACAGATCACAATCTTCTAATGACACTTTTCCA
ACTGTAAGCTTTAGTTTGCATTCTCATTAGTCATTACCAATAATTTTGCATGCTTAACGT
TCATTCAATTGCTTACGGTGTTCGTGTAACCGATTGCTTTTATTTCCTATATGTATGCTC
ACTGCTCTGTCAAGTACTACTTGACGGTCGTATAATTTTGGTTTTGTATAGGTCATGCAC
ATTGCAGCTGCAACCGAGATTACTTCGAGGCTAATCCCTAGTTTGAAAAATTTGCATAGC
TCTTTGGAATCTAAGGTGACTCATTTGATGTAGTCACTAAATTGCAATATTTTAAGATGA
TACTTGCTCTGACCTAAATGGTGTCAGGGAACACTCTTGACCTCAGTTCAATGTGGTACT
ATTTATATTTGGGAGTTGCTTTGTAAATGTTGCAATTTTACATTTAAGGAACATCTTCTG
ACCTTTG

Last Updated on 06.12.2018 16:11