Genotyping and primer details


Genotyping and primer details for 107E05 - At5g50950
Line ID 107E05
Locus At5g50950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20730718 of line 107E05 Chr5:20730718
   
Insertion allele amplicon 8474 - CP05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CP05: 5'-TAACAAAATGACGTCAAAGGTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20731457
   
wt amplicon (over InsPos) 32rd - CP05
wt amplicon start Chr5:20730192
wt amplicon end Chr5:20731457
wt amplicon size 1266 bp
Locus specific primer CP05: 5'-TAACAAAATGACGTCAAAGGTCAG-3'
2nd locus specific primer 32rd: 5'-AAGTAGCAGAAGGAAAGCTCAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11