Sequences from confirmation amplicon


Confirmation sequences for line 107F12 and hit At1g64583 are shown.
Line ID 107F12
Locus At1g64583 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23987427
Primers used for PCR BJ77 - 3144, show primer details
   
Sequence Name 01-K104856-0022-107-F12C-BJ77
GenBank Accession LN486567 [GenBank]
Sequencing primer gene specific primer, BJ77: 5'-CAGAATTCTAAAGATGCAACACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104856-0022-107-F12C-BJ77
TTCTTTCCCCCGGTCAGTAAGTACCACACACTTCAAGCTTCTCCTGACCAAAACCAATCT
ACACAGTCCCCTCATATGACTGTAACAAACAAGCCTAGCTCAGAGTCCCAACAAAAAAAC
GTGACTGGACTCTGATCATATAACATTAGCTGACAAGGCTTATCTCCTTATTACTTGAGC
TATGCTCTTCTAGATGGTCATCTTCCGCATTCATTTGACATATGATCCCCTCCTCTTTCA
TTCTTCTAATCAGCTCATCCGCTTCACGCCTAGGCCCATTCTTACATAAACCTAGTATCA
TTATAGTGTATGTTCTAGCATCAGGCTTCACTCCTTCGACAGGGAGTCTACAAAATAACT
CCCAAGCTTTTCAGGGGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCT
CCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTG
CGTCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 17-K104857-0022-107-F12C-3144
GenBank Accession LN493843 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K104857-0022-107-F12C-3144
TTCGCAGACCCTTCCTCTAATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCCCCTGAAAAGCTTGGGAGTTATTTTGTAGACTCC
CTGTCGAAGGAGTGAAGCCTGATGCTAGAACATACACTATAATGATACTAGGTTTATGTA
AGAATGGGCCTAGGCGTGAAGCGGATGAGCTGATTAGAAGAATGAAAGAGGAGGGGATCA
TATGTCAAATGAATGCGGAAGATGACCATCTAGAAGAGCATAGCTCAAGTAATAAGGAGA
TAAGCCTTGTCAGCTAATGTTATATGATCAGAGTCCAGTCACGTTTTTTTGTTGGGACTC
TGAGCTAGGCTTGTTTGTTACAGTCATATGAGGGGACTGTGTAGATTGGTTTTGGTCAGG
AGAAGCTTGAAGTGTGTGGTACTTACTGACCGGTGGGTAAAGAAGCACTCAAGCATAAAA
CTGCTGCAGAGATGGTGTTGC
Primers used for PCR 13tv - 3144, show primer details
   
Sequence Name 21-K116964-0022-107-F12C-13tv
GenBank Accession LN495826 [GenBank]
Sequencing primer gene specific primer, 13tv: 5'-TGCTCTTCTAGATGGTCATCTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116964-0022-107-F12C-13tv
TTCAATCAGCTTCATTCCGCTTCACGCCTAGGCCCATTCTTACATAAACCTAGTATCATT
ATAGTGTATGTTCTAGCATCAGGCTTCACTCCTTCGACAGGGAGTCTACAAAATAACTCC
CAAGCTTTTCAGGGGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCC
AAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCG
TCATCCCTTACGTCAGTGGAGAT
   
Sequence Name 03-K116963-0022-107-F12C-35St
GenBank Accession LN487650 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K116963-0022-107-F12C-35St
CTTCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATT
GTCGTTTCCCGCCTTCCCCTGAAAAGCTTGGGAGTTATTTTGTAGACTCCCTGTCGAAGG
AGTGAAGCCTGATGCTAGAACATACACTATAATGATACTAGGTTTATGTAAGAATGGGCC
TAGGCGTGAAGCGGATGAGCTGATTAGAAGAATGAAAGAGGAGGGGATCATATGTCAAAT
GAATGCGGAAGATGACCTTAAGAGAAGAGCA

Last Updated on 06.12.2018 16:11