Sequences from confirmation amplicon


Confirmation sequences for line 107G05 and hit At1g50040 are shown.
Line ID 107G05
Locus At1g50040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18543626
Primers used for PCR 04tq - 8474, show primer details
   
Sequence Name 56-K116920-0022-107-G05C-04tq
GenBank Accession LN509215 [GenBank]
Sequencing primer gene specific primer, 04tq: 5'-AATGGTTTGGTGTTTAAATTCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K116920-0022-107-G05C-04tq
CGATAACGATCCATGATCTCCTCCGGTTCACCAGTAGCTATGGCTTCAATGGTCACACCT
TTCGTACCATCTCCTGGTTCCAACCGCGTCTCTCGGTCTAGTCCTGGAGCTTGGCTAATC
CTCAGACCTGATGGCTACACCTGGAAGCCATGGGTTCGCCTTCAGGCATGGCGTGAACCA
GGCGTCTCCGATGTTCTAGGTTACCGTTTCGAGCTTTATAAAGACGGTATCGCTGTTGCA
GTCTCTGCTTCCTCGTCGATCAGCACAAAACTTGGCGGGAGTTTCATCATTGATGGATCC
ACTTCTACGACTACGACTGCTTCTTGGAGTAGCTCTGAAGGAAGTTTCGATCTTTCTTCG
TGGTCAAGCATCAGATCATCAAGAACTGATTCAGGATCGGGGTCGGATTTCAGGTTCTCA
CTGTCTCAAGCGCAACAAAACTTGGGGTTTGTGATGTCGACGAGGGTGCAAGGAGTGGAG
AAACAGAGCAAGCCTAAGGTGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 12-K116919-0022-107-G05C-8409
GenBank Accession LN491755 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116919-0022-107-G05C-8409
ACATCACAAACCCCAAGTTTTGGTTGCGCTCGAGACAGTGAGAACCTGAAATCCGACCCC
GATCCTGAATCAGTTCTTGATGATCTGATGCTTGACCACGAAGAAAGATCGAAACTTCCT
TCAGAGCTACTCCAAGAAGCAGTCGTAGTCGTAGAAGTGGATCCATCAATGATGAAACTC
CCGCCAAGTTTTGTGCTGATCGACGAGGAAGCAGAGACTGCAACAGCGATACCGTCTTTA
TAAAGCTCGAAACGGTAACCTAGAACATCGGAGACGCCTGGTTCACGCCATGCCTGAAGG
CGAACCCATGGCTTCCAGGTGTAGCCATCAGGTCTGAGGATTAGCCAAGCTCCAGGACTA
GACCGAGAGACGCGGTTGGAACCAGGAGATGGTACGAAAGGTGTGACCATTGAAGCCATA
GCTACTGGTGAACCAGAGAGATCATGGATCGTTATCGACCAACCTTTTCTCTCTTTTGAA
AACTGTTCCTTTCCACTTGTCACCGATGATAAGCTGCAGAATT

Last Updated on 06.12.2018 16:11