Sequences from confirmation amplicon


Confirmation sequences for line 107H07 and hit At4g23590 are shown.
Line ID 107H07
Locus At4g23590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12307521
Primers used for PCR 03cv - 8474, show primer details
   
Sequence Name 29-K109029-0022-107-H07C-03cv
GenBank Accession LN499324 [GenBank]
Sequencing primer gene specific primer, 03cv: 5'-AATATCGCAAAAGTGTTCTCGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K109029-0022-107-H07C-03cv
CAAGTCTCTTTCGGGATGAACTCATACCTTCGGACCTCAAGGTGTTTGTAGATAGAGTGG
ACCATGTCCCATGGGAAGCCTGGCCTCGGAAGCAAGATGTTGGCTTTCGGTTTAGCCAGA
ATACTAACCGCGAGCTCGATTGCTTGTTTGCATCCAACAGTCATATATACGTCATCTCCC
GTTAGCTTATTGTCAAGATCTCGGTTTAGATATTCCGCCACAGCACTGATCATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATT
   
Sequence Name 13-K109028-0022-107-H07C-8409
GenBank Accession LN492076 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K109028-0022-107-H07C-8409
ATTTACATTGAATAATGATCAGTGCTGTGGCGGAATATCTAAACCGAGATCTTGACAATA
AGCTAACGGGAGATGACGTATATATGACTGTTGGATGCAAACAAGCAATCGAGCTCGCGG
TTAGTATTCTGGCTAAACCGAAAGCCAACATCTTGCTTCCGAGGCCAGGCTTCCCATGGG
ACATGGTCCACTCTATCTACAAACACCTTGAGGTCCGAAGGTATGAGTTCATCCCTGAAA
GAGACTTTGAGATCGATTTCAACAGCGTTAGAGAGATGGTAGACGAGAACACTTTTGCGA
TAT

Last Updated on 06.12.2018 16:11