Sequences from confirmation amplicon


Confirmation sequences for line 108C11 and hit At1g20823 are shown.
Line ID 108C11
Locus At1g20823 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7239451
Primers used for PCR KP35 - 8474, show primer details
   
Sequence Name 56-K030512-022-108-C11C-KP35
GenBank Accession LN509000 [GenBank]
Sequencing primer gene specific primer, KP35: 5'-TGGCTCAATGCTAGTGACTAATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K030512-022-108-C11C-KP35
TCGTATTCGTCTCACACAAAAAAATCATGAATTTAAACAAACGTAAAGTTATCGAGACAC
TTACTATATAAATGATGTAATGATATAGTATATAATGCATATAAAGTTAAAAATCAATCT
TCATGAAACAGAGGTAACTTCAGCAATTGTTTTTGATAAACACAAAGTAATAAAAGGTTC
AAATACACAAATCGTTAAATTTTCCAATTCATACAACATTTACAAATTACAATAAACCAT
GTACATCAAGAGACTAAACACTATCCCACTAAAATGATCTGCATTAAATTATAGAGTTTT
CTAATAAATAAATGGGAAACTAAAATGATTATGGCAAGAAGGAGTTGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCT
Primers used for PCR KP36 - 3144, show primer details
   
Sequence Name 25-K030513-022-108-C11C-3144
GenBank Accession LN497316 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K030513-022-108-C11C-3144
CTCATCTTCGCCTTGCTTGATTCGGATCTCGATTTCGGGTTCGGATTCAAGTCCGGAGCT
AGAGCTACCGGGTAAACCTCCACACTTGTGACACCTGGCAACAACCAAGATCTGACGGCA
AGAAGGGCAAGACGAGTGAGATCCTAGCCACGTGTCAATGCAAGCCACGTGGAACCCGTG
ACCACACTGCGGTAAAACTCGGAGCTCGTCGCCGGCAGAGAATTCAGCTAGACAGATCGC
GCACTCTGCGAATTTCTCCGACTCAGGTGACTCTGGCGAGAACGTAAGCTTCGGGAGAGA
TTGAAGGACTTTCTTCTTCAGGCCTTTGTTCGCCGCTGCCACCGGAGGTTGAGGAGATGA
AGTTTTG
   
Sequence Name 26-K030513-022-108-C11C-KP36
GenBank Accession LN497794 [GenBank]
Sequencing primer gene specific primer, KP36: 5'-CTCAAACTCAATCTCCTCAACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K030513-022-108-C11C-KP36
GCCGGCGAACGAGCTTCGAGTTTTACCGCAGTGTGGTCACGGGTTCCACGTGGCTTCGCA
TTCGACACGTGGCTAGGATCTCACTCGTCTTAGCCCTTCTTGCCGTCAGATCTTGGTTCG
TTGCCAGGTGTCACAAGTGTGGAGGTTTACCCGGTAGCTCTAGCTCCGGACTTGAATCCG
AAACCCGAAATCGAGATCCGAATCAAGCAAGGCGAAGATGAGTATGAATATGATGGCCAG
TAACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTT
CTTTTTCCACGATGCTCCTCGTGGGT

Last Updated on 06.12.2018 16:11