Sequences from confirmation amplicon


Confirmation sequences for line 108D04 and hit At3g07380 are shown.
Line ID 108D04
Locus At3g07380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2361755
Primers used for PCR 59qi - 8474, show primer details
   
Sequence Name 31-K115550-0022-108-D04C-59qi
GenBank Accession LN500294 [GenBank]
Sequencing primer gene specific primer, 59qi: 5'-ATAGTGAATGATTGTTTGCATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K115550-0022-108-D04C-59qi
TTGATGAGTTTTTCATGTTCCGGTGAAGAGACGATTTCGTCTGTGATGGAATCTTTGGAG
GAATATTTTCAGTTTACTATTGAACTGATGCCTATGAGTAGTCAGGTTTGTTATTCCGGT
GATGGCCCGGCCAGAACTTACAGGTAACATTCATAACTTTGTTGATTTTAATGATTGAAC
GTTAACCAAAGCCCATGTGTTGTTTATTGAGTGACTTAGAATATTAGTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCATCGATATT
   
Sequence Name 04-K115549-0022-108-D04C-oriL
GenBank Accession LN488047 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115549-0022-108-D04C-oriL
CATTTACATTGACTAATATTCTAAGTCACTCAATAAACAACACNATGGGCTTTGGTTAAC
GTTCAATCATTAAAATCAACAAAGTTATGAATGTTACCTGTAAGTTCTGGCCGGGCCATC
ACCGGAATAACAAACCTGACTACTCATAGGCATCAGTTCAATAGTAAACTGAAAATATTC
CTCCAAAGATTCCATCACAGACGAAATCGTCTCTTTCACCGGAACATGTAAAAACTCATC
AACATCAACGAAGAACATCCACTTCGTCATGAATACTTATGCAAACAATCATTCACTATA

Last Updated on 06.12.2018 16:11